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We narrowed to 741 results for: REV

Showing: 361 - 380 of 741 results

  4. Lentivirus Production

    Type
    Protocol
    ... time, allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...mix well. Gently aspirate the media out of the previously seeded 10 cm plate. Slowly pipette the transfection...images; right panels: GFP channel images. Last reviewed on: August 2, 2023...

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge tubes, Neptune 3745...mL polybrene: Dilution Volume of Lentivirus or Previous Dilution (μL) Volume of DMEM Complete Containing...colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

  6. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...includes a step that both fragments the DNA and prevents it from forming concatemers later. The "FS" stands..., one was only able to extract libraries from previously fragmented DNA.The general process for library...

  8. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...for visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools...CRISPR-Sirius (Ma et al., 2018). They improved on previous CRISPR-based loci visualization tools by increasing...

  9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...therapeutics for complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use...Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https:/...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine...inserts and larger targets. The NGS readout also reveals additional information about the presence of non-plasmid...

  12. How to Lead a Great Meeting

    Type
    Blog Post
    ...day in advance. This gives everyone a chance to review the agenda and add anything that is missing.  During...Send around relevant documents, like proposals to review or project plans, before the meeting. At Addgene...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...brightness, photostability, and so on; see the previous blog post for more discussion of these factors...that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent...

  14. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... other fields or writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful...Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online with...

  16. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development... the morning! 9:00 AM--Technical implementation review. The developers discuss which front-end and back-end...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target...
Showing: 361 - 380 of 741 results