...Berkeley at the time and an eLife ambassador, had previous experience organizing open access events and grassroots...Scientific Outreach at Code Ocean and through her previous work at Center for Open Science and Sense about...

...studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these...

...The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell...

...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical signaling ...gradients for control of growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed...

..., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along...

...detecting genome-wide double strand breaks (BLESS), revealed that these mutants do not cause off-target effects...to their enhanced specificity. Additional work revealed that mutations in the mobile Rec3 domain facilitate...

...might need to weigh it out in the fume hood to prevent exposure. Read the reagent’s material safety data...used to contain the material you’re weighing and prevent cross-contamination with other substances. They...spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop out the material...

...peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein of interest...within seconds after a pulse of blue light, and is reversible upon termination of light stimulation. CRY2clust...

...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....number with links to the plasmid pages for your review. We’ll also mail you a deposit kit with instructions...

...about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors...be involved in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also...

...otherwise be lost in lab notebooks forever. microPublications are peer-reviewed and assigned a DOI that can ...

...to light as antibodies became commercialized, revealing the unreliability of antibodies. There are three...reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody crisis states...

...Step 3: Reviewing the plasmid summary on the Fragmid website.   Step 3 is simply a review of the ...

...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

...based methods for viral titration consider using a reverse transcriptase mutant in a separate control reaction...over-inflate results. A packing vector deficient in reverse transcriptase will produce low levels of virus ...

... histones, the more compacted it becomes. This prevents the cellular machinery from physically interacting... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding...

...need to be signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it ...items. This will give your supervisor a chance to review what you have discussed and add suggestions if ...

...insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there...