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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 660 results for: SIM

Showing: 361 - 380 of 660 results

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  5. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

  9. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

  11. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

  14. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

  17. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

  18. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... of interacting and is much more effective than simply sending a message asking how things are going. ...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...
Showing: 361 - 380 of 660 results