We narrowed to 657 results for: SIM

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...

2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ...tiny bit of the sample itself — so easy that this simple procedure is often taken for granted. In this post... from a NanoDrop. The principles will apply to similar instruments from other companies, like the Implen...

3. SciComm: Taking Science to Elementary and Middle Schools

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   ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

4. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...random association between FPs independent of PPIs. Simply compare the fluorescence intensity of your BiFC...whereas BiFC is useful in scenarios that call for a simple, yes-no answer — i.e., do two proteins interact...

5. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...

6. A Guide to Starting Your Own Journal Club

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   ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

7. Great Results Start with Great Standard Curves

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   ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...

9. Plasmids 101: Colony PCR

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   ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...

11. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...and dissemination of experimental protocols, simultaneously allowing scientists to actively comment, annotate...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...

12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...software includes Golden Gate cloning tools that simulate the restriction–ligation reaction. You can input...

13. Designing Your Chalk Talk for the Academic Job Interview

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    ...chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk...chalk talk Perfect your story and delivery. Design simple figures and diagrams to illustrate your vision....

14. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

15. An Inside Look into Addgene's Dev Team

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    ...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would...

16. Plasmids 101: Stringent Regulation of Replication

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    ...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

17. Bioinformatics at Addgene

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    ...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

18. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates.... The optimized plasmid curing method was much simpler and took only ~32 hours instead of ~60 hours. ...

19. Hot Plasmids - November 2023

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    ...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

20. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...