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We narrowed to 634 results for: des.2

Showing: 361 - 380 of 634 results

  1. Our New Antibody Mascot is....

    Type
    Blog Post
    ...some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig. 2: The mascot trio!...

  2. Magnetic Control of Proteins: More than a Dream

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    Blog Post
    ...of ~75% in response to the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled...didn’t need any added cofactors.      Figure 2: Fluorescence intensity and ΔF/F of MagLOV, a variant...Maria were encouraged by previous reports that described magnetic responses of cryptochrome proteins or...

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...retain their individual activities when separated (2), and that DBD and TAs from different systems could... Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation ...activation. Including a prey-only control also provides a baseline level of reporter activation. Vary ...

  4. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...faster when using a dual guide system.   Fig. 2: Size screening for large deletion events.   ... Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our CRISPRGuide...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product...nucleotide along with your desired edit. This silent mutation should be designed solely for restriction enzyme... updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells... your cells were appropriately edited for your desired mutation - insertion, deletion, or site-directed...know a few things about your target cells and the desired edits. First, you will need to know what the ploidy...

  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...sequence is unique compared to the rest of the genome.  2) The target is present immediately upstream of a Protospacer...utilized allowing for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase...vectors and design your sgRNA targeting sequence using one of the many freely available sgRNA design programs...many cases several exons’ worth of DNA. Any ∼20 nucleotides of DNA can serve as the target for Cas9’s single...Cas9 localizes the deaminase to where the edit is desired, and it performs the programmed edit within the...flexibility. A consideration with this tool is that the desired edit will be performed on all relevant bases within...system utilizes an RNA template containing the desired edit adjoined to the sgRNA, a reverse transcriptase...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral...’re delivering their payload to.   Figure 2: Numbers of AAV tools, by category, available from...site, and titer. Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult... for vectors in the “controls” category, which includes things like viral vectors that deliver GFP. ...most requested serotypes (Figure 4), our catalog includes vectors in a variety of serotypes, including the...early engagement with UPenn to our launch of the redesigned Data Hub in 2022. It’s influenced the way we ...viral vector educational resources, from eBooks to guides to protocols, which was why I was so excited to...

  7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post
    ...blood are reprogrammed immediately after collection [2]. PBMCs display unique epigenetic signatures that ...PMID: 27882265. PubMed Central PMCID: PMC5118044. 2. Kim, Y., et al., The Generation of Human Induced ...iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop and use patient-specific...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...the CytoTune™-iPS 2.1 Sendai Reprogramming Kit designed to generate clinical grade hiPSCs is sold at $11,000...replication, OriP and the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two...reported efforts. The pCXLE episomal system thus provides a practical way to generate transgene-free, virus-free...

  8. The Addgene Antibody Data Hub

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    Blog Post
    ...whether the results include knockout data.    Figure 2: Search options in the Antibody Data Hub. Data reports...antibody worked under the conditions described above, and a short description of how the antibody performed....the submitted application, along with a brief description of the experiment. If that data was included ...ELISA — but in all cases, it contains a detailed description of the experimental conditions.     Figure 4...The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate, and...

  9. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...Information Science and Technology 60.4 (2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research...Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools.... After months, or years, of grueling effort to design, fund, and conduct a research project, the vast...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...websites). WeShareScience is a social platform that provides links to the videos, keeping the copyright and...

  10. Addgene's Website Update

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    Blog Post
    ...prompt you to log in or create an account.  Figure 2: The new deposit materials bar New menu Most common...and the Addgene Help Center. A big part of this redesign was pulling all of our science options into a ...Addgene’s website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines...word for it — check out all the new features and designs addgene.org! And when you’re finished, feel free...

  11. Your Top Requested Plasmid in 2015!

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    Blog Post
    ...PMID: 24336571. PubMed Central PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...sampling of the research it has already been used for includes work probing mTOR signaling in cancer cells, deciphering...

  12. 500,000 Plasmids Shipped and Counting!

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    Blog Post
    ...at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around the...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...

  13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... both substantial merit and national importance; (2) The foreign national must be well positioned to advance...outstanding achievements in your specific academic field, (2) that you have three 3 years of research experience..., Casseus Law suggests a mix of experts including 2 dependents –people who have worked with you i.e. your...The recently established case Matter of Dhanasar provides the three-prong test that US Immigration officers.... As Immigration is a federal law, Casseus Law provides legal services to people located in all 50 states...

  14. Inntags: Innovative Protein Epitope Tagging

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    Blog Post
    ...tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system...

  15. Plasmids 101: Stringent Regulation of Replication

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    Blog Post
    ...the chromosome, ending replication.   Figure 2: Prokaryotic chromosomal replication. Adapted from...Microbiology and Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post
    ...efficiencies 2-3-fold. However, the process of double nicking increases indel formation slightly. Designing the... editing as efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime...using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025...site by the pegRNA — a guide RNA that also encodes the desired edit and homology to the genomic DNA locus...both specifies the target site and contains the desired edit (or edits) engages the prime editor protein...and the reverse transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked...edit within what Fyodor Urnov refers to as “PAM deserts” in the genome. More versatile and precise than...

  17. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ...publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene...Specifically this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9...is an Assistant professor at the Biodesign Center for Molecular Design and Biomimetics and the School of...development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided by... toehold switches, which allow for the rational design of RNA sensors that can detect virtually any RNA...areas conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...

  18. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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    Blog Post
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...colleagues describe in the PLoS ONE paper that introduced the kit in December, GreenGate includes the six... bring this into your target organism. We have designed a Golden Gate system based on the daily needs ...and cut another,” Lohmann said. “It allows us to design modules that always have the same recognition site...produced will have different overhangs, they can be designed such that they go back together only one way and...

  19. Let There Be LITE Plasmids

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    Blog Post
    ...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn them on or off...News & Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First,...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...  Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic of Opto-RhoGEF design and activity...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements into many... and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...plasmids if you want to make a stable cell line. This design allows highly localized, rapid and reversible microtubule...processes in cells and tissues. And, this Opto-RhoGEF design could be extended to other GTPases. The Chow Lab...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and...
Showing: 361 - 380 of 634 results