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We narrowed to 508 results for: dna plasmids

Showing: 361 - 380 of 508 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts...origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll...the promoter, which is necessary for getting the plasmid express our gene of interest. This element is responsible...  The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase (RNAP). In bacteria this .... This means that the promoter present in your plasmid backbone must to be compatible with the type of...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism...development-specific promoters available to scientists. Plasmids are oftentimes put to therapeutic uses, and in...

  2. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...Ginkgo Bioworks COVID-19 Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many ...Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration...

  3. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...measure the DNA concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ...transfect plasmid DNA directly into the target cells. The shRNA will be expressed, but the DNA is unlikely...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives...shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral... also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg...#B7001S NEB buffer 2 NEB #B7002S T4 DNA ligase NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha ...

  5. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background Information Plasmids can carry...isolate your plasmid DNA from the bacterial culture by following Isolating Your Plasmid DNA . Antibiotic...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid from bacteria...numbers of bacteria necessary to isolate enough plasmid DNA for experimental use. The following protocol ... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may ...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment... days of lost productivity waiting for follow-up DNA oligos to arrive. We also were careful to set up ...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  7. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can...anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site...cut out the band containing your vector DNA. Gel purify your DNA away from the agarose using a commercially...Modify your plasmid MCS or add short sequences using oligo overlap cloning. Protocols... Protocols Plasmid Modification...Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning You may also like... Restriction...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

  9. p53 Pathway

    Type
    Collection
    ...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription...tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation... negative regulators, increase its stability and DNA binding activity, and allow it to bind transcriptional...of these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues...

  10. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...more than just the DNA sequence. If this is the case, you can confirm your edit using DNA-based methods like...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...mutations or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ...efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters... CD8+ cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...simpler to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual...

  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA ...Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence...system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche,....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver...Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira...PMID: 26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide...

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...curated collections of plasmids as kits with greatly reduced costs per plasmid. We generally create kits...just the tool you need. The plasmids in this portion of the BIOFAB Plasmid Set contain terminators of ...populations. This kit consists of a 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F...researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. ...kits to allow you to easily use a set of plasmids together on a tight budget. To help popularize our ...order from the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit... production (a variety of pExpression vectors) plasmids. The type IIS restriction sites used in these ...

  15. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...of the viral genome and the absence of unexpected DNA contaminants. The data is also used to determine ...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting integrated...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid Size (bp... each plasmid DNA into the bottle containing the Opti-MEM. Mix well. Add 4 mL of PEI (1:2 μg DNA to μg...conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene ...(bp) DNA Concentration (μg/μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer...Transfer Plasmid 5,842 1.00 584.2 Total bp 24,961 In total, we would like 2 mg of DNA or 2000 μg. Using...molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08 μg/bp Therefore, for each plasmid we need: Sample ... the total number of base pairs from all three plasmids, we can determine the total μg/bp we need to achieve...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow...production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...distant cousins of well-known Cas9. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA ... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ....cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova...Our CRISPR Guide Pages Check Out MpAgo from the Doudna Lab Find Validated gRNAs ...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... uses a 3rd sequencing trace file from the donor DNA. This method can precisely estimate editing frequency...clones. The drawback is that you must generate a DNA molecule with homology to your genome edit to mimic...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...

  20. General Transfection

    Type
    Protocol
    ...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...to the diluted DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate ...possible range of ratios to test: Ratio of DNA:PEI Amount of DNA (μg) Volume of 1 mg/mL PEI (μL) 1:1 18.9...protocol to transfect mammalian cells with your plasmid of interest. Protocols...25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...
Showing: 361 - 380 of 508 results