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We narrowed to 879 results for: ids

Showing: 361 - 380 of 879 results

  1. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software ...

  3. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ..., a more stable 130 bp version is used in most plasmids. In an AAV vector plasmid, they flank the gene...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...

  4. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., et al. Adenovirus-Mediated Gene...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various plasmids for overexpression...filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic...Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection...several advantages over existing methods. First, it avoids activating other signaling pathways, which frequently...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, it could be...

  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered...and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  10. CRISPR in the Clinic

    Type
    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...B, Herrera-Carrillo E. A CRISPR-Cas Cure for HIV/AIDS. Int J Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390...ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History ...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...essential components of a virus into different plasmids. For a retroviral system this looks like the genetic...production then proceeds by transfection of all three plasmids into a producer cell line, such as HEK-293T, production... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? Resources...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ....cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ..., a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture...joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested...

  16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...chromoprotein Ultramarine with hydrophilic amino acids to reduce non-specific interactions with cytosolic...research Read our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987...

  17. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

  18. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene   ...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...

  20. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...glycines, serines and other small, non-aliphatic amino acids. However, long linkers increase the chance that ...fusions, our experience is that a linker of 4 amino acids (PVAT, when we use the AgeI site in the multiple...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...
Showing: 361 - 380 of 879 results