We narrowed to 470 results for: mag

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

2. Antibodies 101: Fab Fragments

   Type

   Blog Post

   ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

3. Antibodies 101: Conjugation

   Type

   Blog Post

   ...chemical reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well...

4. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   ...of 2 96-well microplates (identical copies, see image above). We then select forward and reverse primers...

5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   ...sufficient quality (error bars, statistics, clarity in images etc)? Did they perform all of the appropriate controls...

6. Lab Automation at Addgene

   Type

   Blog Post

   ...agar in it. The agar sits on a hot plate with a magnet so that the agar doesn’t solidify while it goes...

7. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   ...single-stranded DNA stretch that is accessible. Image from Gaudelli et al., 2020. Directed evolution...

8. Early Career Researcher Toolbox: Finding Relevant Papers

   Type

   Blog Post

   ...important features such as influential citations, images, and key phrases. It also takes into account how...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   ...Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     Here are ...

10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ... Figure 1: The key components of Nanoblades. Image from Mangeot et al., 2019.   MLV GAG-POL polyprotein...

11. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ...contrast interference (DIC) microscopy and computer imaging processing. The combination of these methods provides...

12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    ...induce non-homologous repair at the site of DNA damage. These nucleases are microinjected into a zygote...

13. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple...

14. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell...

15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...Workflow for high complexity Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools...

16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single...

17. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    ... Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669...

18. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    ...chromosomes and reduces the risk of dangerous DNA damage. If the way DNA is wrapped around histones changes...

19. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    ...They can also be dull and painful to sit through (imagine the entire audience playing Candy Crush or reading...

20. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

    ...glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive...