We narrowed to 478 results for: mag

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   ...for one that can report electrical activity via imaging. They took the light-sensitive protein QuasAr2 ...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...the group, lessons learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional...

3. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   ...functionality are happening and this strengthens our image in the world. Addgene is now as much a tech company...

4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ...experiments, Clift et al. observed no stress or damage upon TRIM-Away, and the electroporated cells re-adhered...

5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   ... Fred Hutch website, social channels and Hutch magazine. Getting pulled in all of these different directions...

6. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein College...

7. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...as possible. We must note that CRISPR is not a ‘magic bullet’: its use will always come with some uncertainty...

8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

9. Antibodies 101: Fab Fragments

   Type

   Blog Post

   ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

10. Antibodies 101: Conjugation

    Type

    Blog Post

    ...chemical reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well...

11. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...of 2 96-well microplates (identical copies, see image above). We then select forward and reverse primers...

12. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...sufficient quality (error bars, statistics, clarity in images etc)? Did they perform all of the appropriate controls...

13. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple...

14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ...Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     Here are ...

15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ...single-stranded DNA stretch that is accessible. Image from Gaudelli et al., 2020. Directed evolution...

16. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    ...important features such as influential citations, images, and key phrases. It also takes into account how...

17. Lab Automation at Addgene

    Type

    Blog Post

    ...agar in it. The agar sits on a hot plate with a magnet so that the agar doesn’t solidify while it goes...

18. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell...

19. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ... Figure 1: The key components of Nanoblades. Image from Mangeot et al., 2019.   MLV GAG-POL polyprotein...

20. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ...contrast interference (DIC) microscopy and computer imaging processing. The combination of these methods provides...