We narrowed to 760 results for: mpo
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TypeBlog Post...mammalian cell lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...
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Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work from... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks..., designer PUF proteins can be fused to other components to lend them many other functions for use in ... -
Reflections and Looking Ahead
TypeBlog Post...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post..., regardless of who is paying for them. More importantly, there’s the opportunity cost of your time; an...gRNAs to knockout genes – are essential screening components, the model system and the assay are just as critical... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...patient data to identify mutations of unknown importance in cancer Previously available methods for investigating...passenger mutations from the “active” drivers is important for the development of targeted therapeutic interventions... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...#ActualLivingScientists). We also discuss the importance of actionability on the Addgene blog and how ...The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...proteins (FPs) are also the foundation of multiple important technologies, including FRET and optogenetics.... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ... -
Science as Art
TypeBlog Post...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could...wider audience for more than just its biological importance. More of Daniel's stunning work can be found ... -
Celebrating Accomplishments in the Lab
TypeBlog Post... lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes. “When PhD...required to achieve success in the lab, it is important to take the occasional breather and acknowledge... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate... what a site offers to their users, and most importantly, allow those users the ability to quickly access... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...studying COVID-19. “Open scientific sharing is important for creating research synergies and accelerating..., scientific sharing will remain an extremely important part of the future of research. To find other ... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been difficult... are blocked by alpha1-AR antagonist. This is important because alpha2-AR can provide inhibitory feedback... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible. ... -
9 Tips to Achieve Success in Academia
TypeBlog Post.... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...synthetize all compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...ve realized my figures are just as important, if not more important, than my writing. Initially I didn...