...simulations and machine learning to develop a predictive understanding of the adenine base editors which can... regulating and propagating life, there was no turning back. While the world of molecular genetics was...This is typically followed by non-homologous end-joining that results in gene knockouts.   Star Trek: Into...locations in the genome. This was soon followed by the adenine base editor (ABE) enzyme in 2017. ABEs rely on ...editor proteins. She is particularly interested in combining the realms of art and science. Follow her at @...

...you’ll probably find lots of different plasmids containing the same protein of interest tagged with different...provide many advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell...covalently linked to derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). This engineered...FlAsH, ReAsH) Few Easy SNAP-tag 182 Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim...Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling tags on different proteins...able to get a plasmid ready-to-use without any cloning at all! As mentioned above, several variants of...

...Getting more scientific and professional training after earning a doctorate Considered a "stepping stone...also more than eager to help you along your path. Learning about their career paths and goals helped me reflect...research opportunities are also fantastic for broadening your experiences! These applications can be highly...years. But college, and science, are places for learning and growth! As you learn more about yourself and...EleanorWang.     References and resources References Canning EA, LaCosse J, Kroeper KM, Murphy MC (2019) Feeling...

...field from a more traditional policy background, learning the science part on the job. Each path to science...implementing the policy within the jurisdiction of the governing body. This type of work can happen at the national... professional societies, and industry through convening activities, formal lobbying, or strategy setting...The “little p” policy is non-governmental work happening at large organizations who have to develop policies... do you do science policy? Many science policy training programs and fellowships have a strong focus on...nation’s largest integrated healthcare system? Defining your why is what will make you a competitive candidate...

...grant this year. These scientists work on projects spanning from the identification of plant compounds and...activities (1). She has developed a fast endophyte screening system and plans to employ the light‐inducible...integration vectors (see for example this yeast cloning kit) she intends to make yeast capable of desaturating... to bacteria and plants. Her research involves cloning cry genes from the soil bacterium Bacillus thuringiensis...bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry...

...glycosidic bond leads to the release of a purine (adenine or guanine) from the DNA. These apurinic sites if in...used for plasmid amplification are endA negative, meaning the gene has been removed/mutated to neutralize...without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on...Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...

...Morehead Planetarium. Similar things are likely happening in your community, and, if they aren’t, think ...motivated more by the desire to help close the widening gap between science and society by connecting ...out how they got where they are. 8)   Look into training from an organization such as COMPASS. Although...their science matter. If you aren’t ready for a training or one isn’t available yet, order a copy of Nancy...University of California, Santa Cruz, has been turning scientists into confident and connected science...

...Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun...powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR...CRISPR/Cas adaptive immune system seems like the winning ticket to ensure prokaryotic survival; however,...Cong et al. and Mali et al. in January 2013 (7, 8). Nine months later, more than 1500 publications describe...

.... My eye was caught by a couple of unusual job openings, although they seemed too far afield at first ...forget: what would it be like? Then I spotted an opening for being an editor at Science magazine. That one...referees. And I am constantly teaching – often explaining a technical piece of research to others outside...of the world’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and...Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

...website in search of plasmids. Now, scientists are beginning to see Addgene as a large data set. Addgene has...database. Through a series of simulations and machine learning, they created a classifier that predicts whether...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...the genes deposited at Addgene were synthetic. But nine years later, over 20% of the genes deposited that...Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA...

...halves (Fig. 1): one containing the microtubule binding domain and the second containing the growth promoting...number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches used in the...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that the ACE ...

...great experimental plan that requires indirect staining of both targets in your specimen. You scour the...Why does isotype matter? Isotype matters when constraining using the indirect method since a secondary ...alternative isotype (purple, mouse). Depending on the cloning process, all constant regions, just the heavy chain...isotype can not be used for multiplexed indirect staining methods since the secondary antibody used will...specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy chain constant...

... 101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...genomic locations. 6. CRISPR 101: Non-homologous End Joining So you know that CRISPR can be used to generate...gymnastics behind DNA repair by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why ...

...Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation)...PALM, also FPALM) (11). For PALM, a small area containing inactive PA-FPs is photoactivated, imaged, and...and new variants are still being engineered. By combining PA-FPS with superresolution microscopy, scientists... Michelle Cronin...

...for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1..., 1978), a measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other...due to tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically...labeling contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which...PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding the chemistry...

...produce custom gRNA-containing lentivirus, we are providing lentiviral preps containing highly requested ... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...expertise as we’ve gotten these services up and running. Both Alex Chavez and John Doench are members of...

...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...to wonder how he has survived this long without downing any dihydrogen monoxide (H2O). Studying science...the same abstract but with the names reversed, meaning that the findings of the study were also reversed...were exhibiting a phenomenon called “motivated reasoning,” the catchall term for a range of neat little...nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin, 108(3), 480. More resources...

...diversity is impressive, and researchers are just beginning to tap the wealth of CRISPR possibilities. To ... are described below. Yan et al.: WYL-domain-containing accessory protein WYL1 increases RspCas13d and...EsCas13d activity, indicating that WYL domain-containing proteins may be broader regulators of Cas13d ...Cas13d enzymes is another win for bioinformatic data mining. As we continue to harness the natural diversity...Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein.” Mol Cell. (2018) pii: S1097...

...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...required components are very similar. While plasmids containing virus-specific elements such as the LTRs or structural...allows it to replicate episomally, essentially remaining an extrachromosomal part of the host genome that...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...which ones could be helpful to you. Interested in learning more about a specific virus? We have you covered...

...particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein containing Reverse...example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin selection...either side of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts... The transcript begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the...