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...CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post.  On the twelfth day of CRISPR, Addgene gave to ...

...model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple knockout...M. Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation...Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models Learn about Homology Directed...

...results. But what is appropriately validated? This post will go over some of the general considerations ...antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications... Antibodies 101: Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding...

...are being up- or downregulated in a system, detect post-translational modifications, quantify protein levels...immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications In addition to protein...abundance, westerns detect changes to a protein’s post-translational modifications such as phosphorylation...

...where you can thrive and do your best work. This post is focused on choosing a lab, but almost all of ...blunders, setting target up to fail, shifting goal posts without telling the target Warning signs while ...Learn about careers in science communication Read posts on mentoring for scientists Resources on Addgene.org...around and accessible for advice and input? Will a postdoc or lab manager be your actual mentor? Is there ...attending career events or being involved in a grad or postdoc association? Will you learn how to run a lab if...a way to grow and learn. Graduate students and postdocs in particular must care about this. The training... than one lab when you choose a grad school or postdoc lab. Having a comparison can be very helpful in...

...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded.... For a more detailed review, check out our blog post: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems...prior to irradiation with UV light (365nm) and that post-UV exposure it showed cleavage activity similar ... the Addgene Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon...

...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to me:...

...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale,... Keyence BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green ...phosphorylation is one of the most abundant forms of post-translational modifications in cells and research...see the detailed plasmid kit page or read our blog post: Quick, Versatile Plant Transgenesis with GreenGate...Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Addgene's First 10 Years Addgene’s Top 10 Blog Posts from 2014  ... in the presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity...

...researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In...!” — Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016,...

...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in ...information, sign up to receive alerts for antibody posts from our blog!  Resources and References  Resources...

...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency...observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair...

... an in-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review ...the other a crRNA array plus a GFP tag. Four weeks post-infection, there was strong expression of Cpf1 and...

...suggests changes in RC2H1 protein expression and post-transcriptional regulation of SM1v1-containing RNAs... by IRE binding proteins, IREB1 and IREB2, which post-transcriptionally regulate iron metabolism pathway...

...continue where the guide left off - namely, a blog post devoted to the other affinity reagents.  Affinity... antibody?  That is the question… or is it? This post isn’t meant to convert anyone to downsize their ...

... subscribe to our CRISPR blog content.   In this post we will cover: Miniature CRISPR-Cas systems SEND...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "... the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages Read more...

...science career related blog posts Read our science communication blog posts Resources on Addgene.org ...widely. The way you communicate your science at a poster is going to vary depending on the conference you... in an academic lab setting, where student and postdoc turnover happens regularly and it behooves everyone... words like this when starting rotations or my postdoc would have simplified the first weeks or months...you discover during grad school or during your postdoc that you enjoy and have a propensity for cross ...

...information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement...cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement...

...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can...leverage this system in new, creative ways. Today’s post will focus on one such strategy--the FLEx switch...

...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...biological phenotype in a scalable way. This blog post will introduce you to this new technology, and guide...