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...potentially signaling a rough road ahead for students interested in leading the process despite the recent advance...students will pursue this newly available avenue. Interestingly, both of these changes share a common thread...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

...integrity of your virus, perform a diagnostic restriction enzyme digest and compare it to some reliable... use the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior...24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141...

...and type of target sites that flox your genes of interest, a powerful FLEx switch can be created for your... considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position...can be employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used...

...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ...

...gradients use dilutions of your sample or protein of interest to understand the dynamic or working range of ...particularly in the sizes around your protein of interest, but you don’t want to run so long your dye front...membrane. If you’re not sure if your protein of interest is over-transferring (going completely through... validated for your protein and application of interest or validating an antibody yourself. But even with...

...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...tools allowed us to detect and capture proteins of interest to better study them in vivo and in vitro, especially... was to recombinantly append their proteins of interest with unrelated peptides that already had high ...only be added to the C-terminus of proteins of interest and proteins tagged with it needed to be chemically...

...transferred from B. subtilis to the bacterium of interest following IPTG induction of conjugation genes ... transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...

...pathway that are of interest to their laboratory. A single mouse click on a gene of interest will direct the...Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing.     References...

...target near the last coding exon of your gene of interest. For ideal use, this gRNA should be cloned into...endogenous promoter and the 3’ UTR of the locus of interest, since the GFP-Puro will be inserted just upstream...nucleases and their applications. It will be interesting to see what new editing possibilities MMEJ can...

...have children but are interested in testing your SciComm skills or are interested in a career in education...Too complicated and I would quickly lose their interest. I won’t claim that I blew their minds, but I ...

...outcomes on viability if cells are arrested too long. Prolonged cell cycle arrest can lead to apoptosis in as... you don’t accidentally change your protein of interest.   Fig. 2: Stop re-cutting with PAM and...

... and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses that can...vector, a transfer vector including the gene of interest, and the plasmid coding for an envelope. Three...LTR promoter to drive expression of the gene of interest. Consequently, the levels of protein expression...

...probability. Continuous inversion of the DNA sequence of interest can lead to poor expression of genes within that...lines that express Cre only in the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand...plasmids and the low concentration of recombinase restricts their expression to individual cells. With this...

...DNA or RNA nucleotides containing a sequence of interest (red) is amplified using Recombinase Polymerase...in complex with crRNA targeting the sequence of interest and an inactivated fluorescent RNA reporter. If...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any RNAs after...

... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...University, University Park, USA. Dr. Molla is interested in precise genome editing in rice and uses base...

...discover new ways to reach our colleagues and other interested audiences quickly and concisely – video might...share any details about their research itself. Pinterest is also a favorite for millions and their visual...decide if reading more about the research is of interest. In his research, Scott Spicer concludes that ...

...isn’t for everyone, particularly if you’re more interested in selling a product than communicating your ...Reddit has a large and active community of people interested in science. You can see some of the conversations...prepared! The mechanics of doing the AMA were interesting. I started watching the questions and preparing...

...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...Lastly, data is our raw product. If we don’t take interest in how it is released, we will be in danger of... publishers and funders. Clearly, it is in our interest to ensure that our data are reproducible and useful...

...necessary for getting the plasmid express our gene of interest. This element is responsible for initiating the...role in determining where and when your gene of interest will be expressed.      The RNA polymerase...results or even kill your cells if your gene of interest is toxic. To combat this, scientists have created...