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We narrowed to 755 results for: rest

Showing: 361 - 380 of 755 results

  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...which may be of particular interest to MoClo users!). If you’re interested in complex Golden Gate Assembly...overhang set by uploading the set with the Type IIS restriction enzyme and thermocycler protocol. It will also...

  3. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...potentially signaling a rough road ahead for students interested in leading the process despite the recent advance...students will pursue this newly available avenue. Interestingly, both of these changes share a common thread...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...integrity of your virus, perform a diagnostic restriction enzyme digest and compare it to some reliable... use the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior...24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...and type of target sites that flox your genes of interest, a powerful FLEx switch can be created for your... considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position...can be employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...gradients use dilutions of your sample or protein of interest to understand the dynamic or working range of ...particularly in the sizes around your protein of interest, but you don’t want to run so long your dye front...membrane. If you’re not sure if your protein of interest is over-transferring (going completely through... validated for your protein and application of interest or validating an antibody yourself. But even with...

  8. Of Myc and Men

    Type
    Blog Post
    ...tools allowed us to detect and capture proteins of interest to better study them in vivo and in vitro, especially... was to recombinantly append their proteins of interest with unrelated peptides that already had high ...only be added to the C-terminus of proteins of interest and proteins tagged with it needed to be chemically...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...transferred from B. subtilis to the bacterium of interest following IPTG induction of conjugation genes ... transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...

  10. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...target near the last coding exon of your gene of interest. For ideal use, this gRNA should be cloned into...endogenous promoter and the 3’ UTR of the locus of interest, since the GFP-Puro will be inserted just upstream...nucleases and their applications. It will be interesting to see what new editing possibilities MMEJ can...

  13. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...outcomes on viability if cells are arrested too long. Prolonged cell cycle arrest can lead to apoptosis in as... you don’t accidentally change your protein of interest.   Fig. 2: Stop re-cutting with PAM and...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...have children but are interested in testing your SciComm skills or are interested in a career in education...Too complicated and I would quickly lose their interest. I won’t claim that I blew their minds, but I ...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ... and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses that can...vector, a transfer vector including the gene of interest, and the plasmid coding for an envelope. Three...LTR promoter to drive expression of the gene of interest. Consequently, the levels of protein expression...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...University, University Park, USA. Dr. Molla is interested in precise genome editing in rice and uses base...

  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...probability. Continuous inversion of the DNA sequence of interest can lead to poor expression of genes within that...lines that express Cre only in the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand...plasmids and the low concentration of recombinase restricts their expression to individual cells. With this...

  18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...DNA or RNA nucleotides containing a sequence of interest (red) is amplified using Recombinase Polymerase...in complex with crRNA targeting the sequence of interest and an inactivated fluorescent RNA reporter. If...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any RNAs after...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...discover new ways to reach our colleagues and other interested audiences quickly and concisely – video might...share any details about their research itself. Pinterest is also a favorite for millions and their visual...decide if reading more about the research is of interest. In his research, Scott Spicer concludes that ...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...isn’t for everyone, particularly if you’re more interested in selling a product than communicating your ...Reddit has a large and active community of people interested in science. You can see some of the conversations...prepared! The mechanics of doing the AMA were interesting. I started watching the questions and preparing...
Showing: 361 - 380 of 755 results