We narrowed to 23 results for: ccdB

1. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   ...plasmid containing the lethal gene. An example is the ccdB gene system commonly used in Gateway® Cloning. There...

2. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   ...coli fertility plasmid that also encodes a toxin, CcdB, and the resistance to that toxin, CcdA. Knowing...

3. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   ...a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating...

4. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ...strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids).  We also like to...

5. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   ...and distribution at Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of plasmids contain...

6. CRISPR Plasmids - Empty gRNA Vectors

   Type

   Collection

   ...60244 Mammalian Gibson none S. pyogenes Zack pHL-H1-ccdB-mEF1a-RiH 60601 Mammalian BamHI/EcoRI none S. pyogenes...53121 Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral...

7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

   Type

   Collection

   ...lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector...

8. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   ...the desired vector. The pCoofy vectors contain the ccdB cassette, which is not copied during the PCR amplification...

9. Gamma-Retroviral Vector Guide

   Type

   Guide

   ...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...

10. Adenovirus Guide

    Type

    Guide

    ...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...