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We narrowed to 62 results for: gfp lentiviral vector

Showing: 31 - 60 of 62 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow...CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Firefly hPGK Lentiviral expression of firefly luciferase Paul Khavari 109395 EFS-Vector Firefly EF1α ...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the presence of...promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly luciferase fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from... the same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of...

  5. Lentivirus Plasmids

    Type
    Collection
    ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...table lists lentiviral transfer plasmids that can be used with 2nd and 3rd generation lentivirus technologies...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    ...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...methylation and activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, ...tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral transduction. Lysine-specific Demethylase...Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds ...Tet-dependent (TetO-dCas9-D3A) constructs. For lentiviral expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP... targeted enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian...and this fusion allows for packaging into viral vectors and targeted delivery into hard-to-manipulate cell...targeting to a wider set of genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system...

  9. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional...highlights from the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse...the new selection of ready-to-use AAV9 and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available...your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff/Fon-sRGECO ...circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic receptor DAD2. SLs ...which modulates the fluorescence intensity of the cpGFP without altering that of the internal control (Fig...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Vectors - Includes tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial...bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression SGFP2 495 512 ...Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast...Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1 - Mammalian...then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set ...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...you made to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer...enter "Feng Zhang (Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...- #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA... 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...interest (analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could ...plate reader, or any other instrument which measured GFP levels and converted that signal into a readable ...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series of HEK 293T cells expressing...membrane-targeted SuperPLDx30 (magenta) and PA probe GFP-PASS (green). After 30 minutes of blue light illumination...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...

  15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...of producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide to Ebola Contribute... viral vector...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene...

  16. Adenovirus Plasmids

    Type
    Collection
    ...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are ... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...regions present in pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral A block for the AdenoBuilder genome assembly...system. Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle ...

  17. Retrovirus Plasmids

    Type
    Collection
    ...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro.... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression...Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional...of dsRed and the activation of your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV...

  18. Plan Your Experiment

    Type
    Collection
    ...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...

  19. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et...like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9...real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence...involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy or...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors... Protocol Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter ...these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol...

  21. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc...Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...

  22. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...

  23. CRISPR References and Information

    Type
    Collection
    ...cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2...CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles ...positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...at Addgene Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive...

  24. Neurodegeneration Research Collection

    Type
    Collection
    ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17. ...motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  25. CRISPR Guide

    Type
    Collection
    ...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  26. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  27. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...Much like lentiviral vectors, gamma-retroviral vectors integrate at random locations throughout the genome... for lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

  28. Plan Your Experiment

    Type
    Guide
    ...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...

  29. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...

  30. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's...21190 GFP-pcDNA3-PKCgamma-cys1Acys1B PRKCG GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma...PKCgamma PRKCG GFP CMV Spinocerebellar ataxia 26 Tobias Meyer 21205 GFP-C1-PKCgamma-C1A PRKCG GFP CMV Spinocerebellar...-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's... GPD 25QDProGFP p416 HTT GFP GPD Huntington's Susan Lindquist 15569 GPD 104QDProGFP p416 HTT GFP GPD Huntington's...GAL 25Q+ProGFPp416 HTT GFP GAL1 Huntington's Susan Lindquist 15581 GAL 46Q+ProGFPp416 HTT GFP GAL1 Huntington's...GAL 72Q+ProGFPp416 HTT GFP GAL1 Huntington's Susan Lindquist 15583 GAL 103Q+ProGFPp416 HTT GFP GAL1 Huntington's...
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