We narrowed to 912 results for: ire;

...including modifying other sites in the genome and directly interacting with transcription machinery. ...to overlap the CpG of interest. Having the CpG directly inside the gRNA sequence is a great guarantee ...

...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro:...pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro) are also in the...M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor ...

... Communications. 2017. PubMed PMID: 28924139. FIRE-Cas9 for inducible interrogation of the epigenome...Article contributed by Joanne Kamens Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed...Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches to enable rapid...cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed to target multiple...analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found here. Braun et al. Nat...controls expression of transcription factor 2, which directly controls expression of a reporter protein, it ...

...)-mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D...Serotype Name 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase AAV Finally, we are excited ...Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Chemogenetics... Serotype Name 119472  AAV5  AV SYN PSAM4 GlyR IRES EGFP   Biosensors Plasmid Serotype Name ...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Control AAVs...

...GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV SYN PSAM4 GlyR IRES EGFP...-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium sensors...released so far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...neurotransmitter in vertebrates, but methods for directly imaging GABA have been limited. Like calcium sensors...

...Element (IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound by IRE binding...cannot distinguish between proteins that directly or indirectly interact with the bait. Also, RaPID can...binding proteins, IREB1 and IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts...interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions...RNA-protein interactions due to sample processing. Require prior knowledge that the protein of interest interacts... methods are not ideal for in vivo work. BirA* requires 16-18 hours to sufficiently biotinylate interacting...found that all HHCS-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences...

...combination of wild type alleles, NHEJ-repaired alleles, and the desired HDR-edited allele. Therefore, it is...presence of the desired edit experimentally and to isolate clones containing the desired edit (see: Validate...) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired edit is incorporated...transcription-activator-like effector nucleases (TALENs) required scientists to design and generate a new nuclease...the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout assay are simple. You’...continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches matters...of the PAM sequence. The resulting DSB is then repaired by one of two general repair pathways: the efficient...

...BioRender.com. Select Your Desired Genetic Manipulation Different genetic manipulations require different CRISPR...percentage of DNA breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base ...methods and often require screening a larger pool of cells to find one with your desired edit. Lastly, you...base edits Can be paired with restriction enzymes for further customization Usually paired with a sequencing...must be experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, ...or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared...Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location, location...

...and study of stem cells, from iPSC generation to directed differentiation....the body and have the capacity to give rise to an entire organism. iPSC technology removes the possible ...below. Once iPSCs have been created they can be directly differentiated into specific somatic stem cells...Plasmids below. Alternatively, it is also possible to directly differentiate one differentiated somatic cell ...endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research Plasmids . For...lentiviral plasmids A drug-inducible system for direct reprogramming of human somatic cells to pluripotency...Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic ...

...antibodies? Will you be using a direct or indirect detection method? If indirect, do you already have secondary...signal amplification through the indirect detection method. In the direct detection method, the signaling...most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein of...antibody methods, it can be done using either an indirect or direct method. Since IF uses fluorophore color to...cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires single-antibody...complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically more expensive... Polyclonal antibodies are typically collected directly from animals and contain antibodies targeting ...

...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru... AAV for Cre recombinase expression in striatal direct pathway D1-MSNs Jonathan Ting Viviana Gradinaru...

... 105829 pIRE4-Azi Azi-tRNA synthetase (EAziRS) p-Azido-phenylalanine (Azi) Mammalian TAG Irene Coin 105830...modifications to the standard translation machinery are required: The ncAA is generally supplied in the culture...these sets have been mutated and screened through directed evolution to charge the tRNA with a different ...-like click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644 pRF0G-Tyr tyrosyl-tRNA synthetase M....182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA.... Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to...

...contains a special Protospacer Adjacent Motif (PAM) directly after where the crRNA would bind. (5) After the...double-strand break (DSB) induced by Cas9 could be repaired through non-homologous end joining (NHEJ) , an...homology arms was supplied, the break could be repaired according to this template, allowing for precise...tags, or loxP/FRT sites, among others. Homology-directed repair (HDR) allows us to create a variety of ...occurred at a truly astonishing pace, with work directed towards increasing on-target editing rates and...cuts only one strand of DNA and can be used with paired gRNAs to lower off-target cleavage frequency. Truncated...rely on NHEJ or HDR: Base editors : Base editors directly convert bases without creating a double-strand...

...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat Commun... with peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based...cellular locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and ...11/520/eaah3738. Justin Blau cAMP (cyclic AMP) cAMPFIRE cAMP biosensors with improved sensitivity Sensitive... PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse fluorescent biosensor for citrate (Citron...mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors...mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive Serotonin...

...Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C....characterized which allow researchers to express desired constructs in precise cell types and/or developmental...plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert ...modified approach has also been developed by the Fire laboratory and described in Efficient Marker-Free...vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without PATC, which diminishes...

...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...can continue for many kilobases in one or both directions from the target region, effectively turning Cas3... Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. ...other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3...

...cultures, these plasmids direct the production of viral vectors that contain the desired genetic cargo. In turn...their mild immunogencity. These viral vectors can direct long-term transgene expression, but generally don't..., such as different promoters or tags, can even direct delivery of the genetic cargo to specific cell ...prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid system....

..., is regression of previously acquired skills, notably loss of acquired purposeful hand movements and ...Envigo. The following cell lines are available directly from the labs in which they were created. Find...rsrt.org The following cell lines are available directly from the labs in which they were created. Find... a p.R168X or p.T158M MECP2 mutation, and the direction and degree of skewing of X-chromosome inactivation...

... Expression T-Sapphire 399 511 26 4.9 1.3 h Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast...Yeast Expression mT-Sapphire 399 511 26 4.9 2.6 h Monomer (A206K) mT-Sapphire-N1 - Mammalian Expression ...Expression mT-Sapphire-C1 - Mammalian Expression mT-Sapphire-pBAD - Bacterial Expression LSSmGFP 400 510 18 4.7...

...removing the amplification and extraction steps required when one receives plasmids in bacterial stabs....yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning...DNA is designed for use in cloning applications. Direct transfection into cells is not recommended. Can...independently including the check out and will require separate shipping charges. Where can I find cloning...

...for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 37825...37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about...

...Michael Davidson 54571 54856 mT-Sapphire-pBAD tdTomato-pBAD mT-Sapphire (donor) tdTomato (acceptor) FRET...peptides are used to enhance protein solubility and to direct recombinant proteins to the periplasmic space between...expression from that element. When this activation requires a transcription factor to bind to a small molecule...pMV306hsp+FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson ,...

...(PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...that binds to a specific genomic DNA locus and directs the nickase to the target site. The longer pegRNA...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription...

...cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (...expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral... Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence...Control Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma...

... and TniQ). Some type I CASTs can result in bidirectional insertions — meaning they may not be suitable...However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end closest to the target... I, type V-K CASTs appear to exhibit almost unidirectional insertions, but are more prone to off-target...

...Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector...

...charges may be required before your order will be delivered. The courier will contact you directly regarding...additional customs documentation that might be required by your country for the import of biological research...

...library expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse ...

..., pmScarlet3_C1 mT-Sapphire* tdTomato 399 0.6 581 138,000 0.69 6.4 5.9 mT-Sapphire-N1 , tdTomato-N1 CyOFP1...self-labeling tags on our blog . Luciferase can be paired with a fluorescent protein acceptor for Bioluminescence...

...doi.org/10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006) Retrograde...–49 . https://doi.org/10.1038/nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann...Infection of these cells with VSV-G pseudotyped virus requires the upregulation of LDLR expression using other...neurons. However, VSV-G’s broad tropism can be undesired for in vivo studies since there’s the potential...neuronal cells and can travel in a retrograde direction from the synapse to the cell body, making it a...use rabies virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies... system (Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with...