We narrowed to 72 results for: rho
-
TypeCollection...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...
-
Using AAV for Neuronal Tracing
TypeBlog Post...Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are...Vidal-Sanz M., and Aguayo A.J. (1987). The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and ... -
15 Hot Plasmids from 2017
TypeBlog Post...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv, et al. Nat Methods. 2016. PubMed... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...interneurons Isocortex 223971 pAAV-3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA AiP12040 AiE0354m FlpO Vip interneurons...interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2-WPRE3-BGHpA AiP11712 AiE0219h SYFP2 Vip interneurons...interneurons Isocortex 208401 pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA AiP12039 AiE0354h SYFP2 Vip interneurons...interneurons Isocortex 163503 pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA AiP11633 AiE0140h SYFP2 Pvalb...interneurons Isocortex 230099 pAAV-hsA2-AiE0086m-minRho-SYFP2-WPRE3-BGHpA AiP11580 AiE0086m SYFP2 Pvalb...interneurons Isocortex 230807 pAAV-hsA2-AiE0086m-minRho-iCre(R297T)-BGHpA AiP20171 AiE0086m Cre Pvalb interneurons...striatum Striatum 191717 pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA AiP12514 AiE0367h_C2 SYFP2 Medium... -
Optogenetics Plasmid Collection
TypeCollection...opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that can ... as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase... -
Brain Initiative Collection
TypeCollection...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond... pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted... -
AAV Viral Preps
TypeCollection...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Yulong Li ADP Biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles... -
New Viral Vectors - Spring 2025
TypeBlog Post...far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ... -
Newly Updated AAV Data Hub!
TypeBlog Post...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post..."Optical control of zebrafish behavior with halorhodopsin." Proceedings of the National Academy of Sciences... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...trophies will serve as badges of honor for our powerhouse depositors who have generously shared their reagents... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post... channelrhodopsin... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the... -
Celebrate National Book Month with Addgene
TypeBlog Post...spectrum. I recently presented Kurt Vonnegut’s Slaughterhouse-Five, and the responses ranged from “one of... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...Cas9’. The Cas9 nuclease is a genome engineering powerhouse, and for good reason. It is guided by an RNA ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening ... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...and BioID, any protein that’s within a ~10nm neighborhood of the bait protein will be biotinylated. This... -
Hot Plasmids - October 2020
TypeBlog Post...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-... -
Plasmids 101: FLEx Vectors
TypeBlog Post...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...Automation and Screening (SLAS) Electronic Laboratory Neighborhood. Work/life balance. Is it truly possible to... -
Hot Plasmids Spring 2024
TypeBlog Post...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral Vector Pseudotypes: Precious... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold brighter... -
Plasmids 101: Repressible Promoters
TypeBlog Post...//doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Cumate-inducible gene expression...