Skip to main content

We narrowed to 6 results for: rho

Showing: 1 - 6 of 6 results
  1. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...fall under two general categories: rho-dependent and rho-independent. Rho factor is a helicase which assists...polymerase in the termination of the transcript. Rho-dependent terminators are not usually employed in...Nearly all common bacterial expression plasmids use Rho-independent terminators, which include naturally ...engineered high-efficiency terminators such as T0. Rho-independent termination is also known as intrinsic...conserved secondary structure and sequence conserved Rho-independent termination annotation for 90 bacterial...structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes. This...Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas Polymerase II termination...
  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...movement, and more, often driven by Rho family signaling pathways. Rho guanine-nucleotide exchange factors...factors (GEFs) activate their partner Rho GTPase, leading to behaviors like lamellipodia or filopodia formation...morphological changes, depending on the specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of...GEFs ITSN1, TIAM1, or p63RhoGEF (which activate the Rho GTPases Cdc42, Rac1, and RhoA, respectively) using...spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic of Opto-RhoGEF design...membrane-recruitment optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems...developed a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022)....
Showing: 1 - 6 of 6 results