Plasmids 101: Terminators and PolyA signals
Type
Blog Post
...fall under two general categories: rho-dependent and rho-independent. Rho factor is a helicase which assists...polymerase in the termination of the transcript. Rho-dependent terminators are not usually employed in...Nearly all common bacterial expression plasmids use Rho-independent terminators, which include naturally ...engineered high-efficiency terminators such as T0. Rho-independent termination is also known as intrinsic...conserved secondary structure and sequence conserved Rho-independent termination annotation for 90 bacterial...structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes. This...Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas Polymerase II termination...