We narrowed to 174 results for: tub

... — check out Addgene’s new Quick Tip series on YouTube! In this series, we share quick tips to help you...video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, ...

...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...

...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...

...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...

... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...

... your final tube after purification. How do you know if you actually have DNA in your tube without seeing...

...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...

...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...

...tested whether or not FP-tubulin fusion proteins could properly form microtubule networks in cells. Other...

...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...

...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...

...Markers : Antibodies targeting popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience...

...prescribed order. These fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively... per cloning reaction drops dramatically, as one tube of enzyme should be enough for at least 50 cloning...

... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...

...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...

...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...

...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...

...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...

..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...

...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...

...Therefore, the localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization...Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction enzymes...

...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang... using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https:/...

...Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor...

...3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top...

...Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's Biosafety Resources Addgene Protocols...

...buffer called sheath fluid and transported through tubes or capillaries to a laser. As the cells move through...positive for a desired parameter is collected in a tube or plate, while a droplet containing a negative ...

...Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L mutation. 1...

...plastic in spin columns and buffer bottles Waste tubes are made of 100% post-consumer recycled plastic ...buffers and columns sold separately Some, waste tubes sold separately No Other Minimized amount...

...with all of the plasmids in the library in a single tube. The number of unique plasmids in a pooled library... submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial ...

...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...