We narrowed to 109 results for: lentiviral dna vectors

...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream... regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression...Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV... Firefly EF1α Lentiviral expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...

...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used ...MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of building blocks...

...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...synthesize complementary DNA copies of their genome upon infecting a host cell. This viral DNA (also called proviral...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...proviral DNA) is then inserted into the host's genome and is used to make more virus. The most studied ...Adeno-associated virus (AAV) is a small, single-stranded DNA parvovirus. AAV can infect humans and some other ...can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning it does...Adenovirus Adenovirus has a linear, double stranded DNA genome which is non-enveloped. It can infect humans...

...(the most popular form), this viral vector is used to deliver DNA encoding Cre recombinase under the control... and other viral vector tools in the years to come. We’ll make many more viral vector tools and resources... Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...be sure to check back often! To find more viral vectors available through Addgene, be sure to check out...out our viral service page and browse our viral vector blog posts for many more tips and ideas on how to...thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional Resources AAV Titers: Where do they...

...consists of a pool of lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are ...individually label millions of mammalian cells through lentiviral infection and to monitor their abundance and ...optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later, Bhang says, “When... Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources...infection. When using the ClonTracer system, lentiviruses containing these barcodes infect the target ...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation... as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound... fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached...4X CDRE repeat. In addition to showing that this vector is suitable for flow cytometry and fluorescence...alternative to intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation, these probes...

...the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell ...physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by lentivirus, which has.... This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific...their optimized vectors to highlight a key advantage of CRISPRa over traditional cDNA overexpression: ...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented by ...

...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol...polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...the diluted DNA mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL of 1 mg/mL PEI 1:1 18.9 18.9 1:2 18.9 37.8...using μg total DNA to μg PEI ratios of 1:1, 1:2, 1:3 and 1:6. The 1:2 and 1:3 total DNA:PEI μg ratios provided...

.... Viral vectors undergo DNA rearrangements. Depending on the composition of the viral vector and the ... about Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages...hepatitis B virus DNA and hepatitis C virus RNA quantification as measured with branched-DNA technology." ... one point or another. After amplifying a viral vector, collecting virus, and transducing target cells...the repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication...genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize rearrangements...have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...

...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...of recombinant DNA technology has allowed molecular biologists to fuse fragments of DNA together in a ...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow...CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly...

...aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA Purification Kit instructions...., the lentiviral particles are serially diluted and used to transduce HEK293T cells. Genomic DNA is extracted... of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence... (ddPCR) for lentivirus vector titration. Protocols... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration... use droplet digital PCR (ddPCR) to titer lentivirus vectors. This protocol specifically uses primers ...Thermo Scientific, 10199-452 Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue...

...derivatives. Unmodified Cas9 causes double stranded DNA breaks that commonly result in a complete loss of...Brunello CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While...These mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to...the Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library ...tracrRNA was combined with the sgRNA expression vector allowing the screen to be conducted with only two...function. Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench...viability genes as they are subject to an increase in dsDNA breaks that can cause cell death. Thus, CRISPR inhibition...

...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article ...Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On...TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene ...Aebischer , Didier Trono 41393 pCW57.1 Lentiviral Tet-On vector for inducible expression; insert with ...Tight TRE David Root 100521 pCW57.1-MAT2A Lentiviral Tet-Off vector for repressible expression with blasticidin...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was relatively...that Surveyor nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to ... denature and renature the dsDNA. Upon renaturation, you will generate DNA duplexes with one strand from...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...gRNA-transduced Cas9-A549 cells are used as a control. DNA was analyzed by PAGE. The basis of the Surveyor...these mismatches are indicated by the presence of DNA cleavage products. The appearance of the 428 bp band...

.... At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and they... better vector for ocular gene therapy: Adeno-Associated Virus (AAV) While other viral vectors such as...gene can be targeted to RPE cells using a lentiviral vector and a clinical trial is ongoing. Moving forward...AdV) and lentivirus (LV) are known to transduce retinal cells, AAV quickly became the vector of choice...technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative...tolerate the presence of antigens (such as viral vectors) without triggering a destructive inflammatory ...retina but only transiently. However new viral vectors capable of providing long-term gene expression ...

...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse...Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused ...Targeting Type VI CRISPR systems target RNA, not DNA, across multiple systems. RNA Editing Cas13 enzymes...

...producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as ...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing...paper for more information Miguel Sena-Esteves pcDNA-SADB19L Contains rabies viral RNA polymerase; ...

...roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within... overviews of three split vector strategies plus one additional split vector strategy for increasing the...homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the...copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp... hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector, but significantly...ideal split vector system. Overall the efficiency of transgene expression from split vectors has been inconsistent..., Duan D (2008) A Hybrid Vector System Expands Adeno-associated Viral Vector Packaging Capacity in a Transgene-independent...

...known or suspected to be involved in DNA damage response and DNA repair.  A new CRISPR pooled library ...only required three components: a master mix, the DNA of interest, and cell extract.    Graphical...They allow single-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...Baradaran’s expertise in viral safety, gene therapy vector development, vaccine development, and assay technologies...she says. In 11th grade she heard a talk about Z DNA that inspired her to major in molecular biology in...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...Melina Fan and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy....

...available as pooled plasmid DNA. In addition, several libraries are offered as lentiviral preparations to save... the pMuSIC library, or by sequencing the unique DNA barcodes. Figure 1: Schematic of barcoding...Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries Pooled Library ...

...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits... two formats: as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...

... safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids ...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...lab. One safety feature of lentiviral vectors is that the components necessary to produce an infectious ...Viral Vectors 101 eBook Biosafety Resource Guide Addgene blog: Tips for Titering Your Lentiviral Preps...transgene expression. Wild-type lentiviruses have been engineered into viral vectors for safe use in the lab....Addgene lentiviral packaging, envelope, and transfer plasmids Viral...

...protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...conduct your custom experiment. Fly vectors The vast majority of fly vectors are based on p-element, random... to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our...

...pandemic, the Krogan lab at UCSF generated lentiviral vectors expressing SARS-CoV-2 open reading frames...which all but one are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker...(2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited...qPCR and antigen tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had already developed...

...macromolecules used in scientific research such as DNA or protein. It comes as no surprise then that stability...Base modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget...ribonucleotides for deoxynucleotides. A partially DNA gRNA is surprisingly well-tolerated by the Cas9 protein....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...of producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide to Ebola Contribute... viral vector...of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication...

...) ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions) DNase-seq DNA (regions that are accessible... regions of DNA that demonstrate “spooky action at a distance.” Through the marvel of DNA compaction, ...details on DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family...See below for further details. DNA is a 3D Fractal Figure 2: DNA compaction. Graphics courtesy ...For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome, ten...coordinates for genetic elements like protein, RNA, and DNA (Table 1). As with GPS in the real world, coordinates...

...  The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase (RNAP). In bacteria this ... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

...connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of DNA parts ...Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a ...background MoClo is a clever way to assemble several DNA parts in a specific orientation using Type IIS restriction...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...assemblies or that compare the effects of different DNA elements on the behavior of an expression construct...

...- #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9... 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...

...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

...integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of...either introduces a ssDNA nick, a dsDNA break, or is engineered not to cleave DNA and instead activates...an experiment. For CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target...genomic locus must often be proximal to a PAM site for DNA cutting to occur. The RNA sequence and PAM site confer...more. While such software typically only considers DNA sequence and doesn’t take into account chromatin ...only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments...generated near each other, this will result in a DNA double strand break (DSB), the event required for...

...intermediary DNA replication step between DNA and mRNA. DNA genome replication can involve an RNA or dsDNA intermediary...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...Louten, 2016 Viruses with DNA genomes can either follow central dogma (gDNA to RNA to proteins) or require...

...incorporate new information into DNA. This also requires an exogenous DNA template. HDR, however, is a very...so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C...made it easier than ever both to engineer specific DNA edits and to perform functional screens to identify...true" %}{% raw %}"Browse Empty gRNA Expression Vectors"{% endraw %}{% end_module_attribute %}{% module_attribute...href":"https://www.addgene.org/crispr/empty-grna-vectors/","type":"EXTERNAL"},"open_in_new_tab":true,"no_follow...efficacious to target the coding or non-coding strand of DNA. As a rule of thumb, we avoid target sites that code... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive...

...in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et...like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9...

...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More Antibodies...Nat Methods. 2024) Base edit human mitochondrial DNA using mitoBEs . (Yi et al. Nat Biotechnol. 2023) ...more. Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...

...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...2017, we had distributed 1,000 ready-to-use viral vector preps. Today, that number has grown to over 114,600...preps. And in 2025, we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I ...research community once more. Our ready-to-use viral vectors were useful for researchers wanting high quality... plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came ...

...fragments of DNA from the helper plasmids or the cell genome can be packaged inside the vectors during production...sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted from... common to find DNA from the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids...mixed another AAV vector with AAV-68544 to validate our method. We then extracted the DNA and processed the...used to identify DNA contaminants in AAV preparations and provide information on these DNA species with exquisite... (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus...blog Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We...

...(scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid...adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy...Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources ...designed so that the single stranded DNA folds back on itself to become dsDNA in the nucleus and is a good option... Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene....Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid...repeats – the sequences that flank the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs...