Skip to main content
Addgene
Showing: 51 - 100 of 161 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...

  14. AAV Viral Preps

    Type
    Collection
    ...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...

  15. Antibody Production

    Type
    Collection
    ...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  16. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ...

  17. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...

  19. Deck the Lab 2022!

    Type
    Blog Post
    ... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...

  21. Rett Syndrome

    Type
    Collection
    ...Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link opens in a new window...

  23. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...

  24. Deisseroth INTRSECT Collection

    Type
    Collection
    ...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...

  27. Editor's Choice, September 2016

    Type
    Blog Post
    ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...

  30. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

  34. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...

  36. Plasmid Cloning by PCR

    Type
    Blog Post
    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  47. Plasmids for Stem Cell Research

    Type
    Collection
    ...fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol...

  49. Hot Plasmids - October 2020

    Type
    Blog Post
    ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

  50. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...
Showing: 51 - 100 of 161 results