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We narrowed to 151 results for: 199

Showing: 21 - 40 of 151 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...5% to 50% per freeze thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the virus will be used ...transduction efficiency by 1.5-fold (Stockschläder et al., 1999). 5. Check your packaging cells for infection If...technology." Journal of clinical microbiology 37.6 (1999): 1683-1686. PubMed PMID: 10325307. PubMed Central...normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID: 2339059. PubMed Central...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...over 25 years ago (Douglas and Howard, 1991, Bubeck et al., 1993). But why did this technique fail to gain... polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed PMID: 2003926. 2. Bubeck, Peter,...recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed Central PMCID...

  3. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid increases, increased...in plasmids that contain them (del Solar et al., 1998). There are two models for iteron-based plasmid ...when plasmid copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative..., Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control of Circular Bacterial Plasmids...

  4. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification systems...Proceedings of the National Academy of Sciences 93.12 (1996): 6059-6063. PubMed PMID: 8650219. PubMed Central...plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451-3454. PubMed PMID: 7768854. PubMed Central...overlapping mok gene." Journal of molecular biology 223.1 (1992): 41-54. PubMed PMID: 1370544. Morin, Cara E., and...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous variants have... D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type 1 integrase ... 721–728. https://doi.org/10.1128/JVI.70.2.721-728.1996. PubMed PMID: 8551608 PubMed Central PMCID: PMC189872...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...EGFP), a variant with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate... reaction that generates the chromophore (Tsien, 1998). Importantly, this reaction requires molecular ...in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum...Tsien, Roger Y. "The green fluorescent protein." (1998): 509-544. PubMed PMID: 9759496.  3. Buckley, Anthony...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...corresponding movie came out in 1993 and sequels of Jurassic Park (1997, 2001, 2015, and 2018) were highly...Jurassic Park by Michael Crichton was published in 1990, just a year after the discovery of meganucleases...dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring Ethan...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang...Roger Tsien and co-workers (Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio...protein molecules inside live cells." Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs...Proceedings of the National Academy of Sciences 96.5 (1999): 2135-2140. PubMed PMID: 10051607. PubMed Central...

  9. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed Central...preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580–6584. PubMed PMID: 1631159. PubMed Central...Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering...

  10. Of Myc and Men

    Type
    Blog Post
    ...domains of Myc1-9E10 in the late 1990s (Fuchs, et al. 1997; Schiweck, et al. 1997) with the goal of making the...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...something that cannot be learned from textbooks” (Brian, 1996). But is it possible to bring the kinds of transformative...type of exercise Einstein might have resented!). In 1997, Joseph Provost, then a new faculty member at Minnesota...Life Sciences Education. 13(4):602-606. Brian, D. 1996, Einstein: A Life. Chapter 17, Einstein Discovers...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile option...addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen ...

  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...that do not express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the envelope and... U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-, and spumaviruses...–6178 . https://doi.org/10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...labeling tag, from Roger Tsien’s lab in 1998 (Griffin et al., 1998), this 15 amino acid peptide contains....2008.01.007 Griffin, B. A., Adams, S. R., & Tsien, R. Y. (1998). Specific Covalent Labeling of Recombinant Protein...

  15. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... lower-prestige journals has risen steadily from 1995 to 2013. Considering citations to all articles, ...non-elite journals went from 27% of all citations in 1995 to 47% in 2013 – yes, almost half.   The effect ...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine MS, McKenzie A 3rd, ...Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ... may have different binding properties (Morelock 1994). While in the majority of cases the differences... R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype choice for chimeric antibodies affects...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796. PubMed...vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. PubMed PMID: 7798275. Additional...
Showing: 21 - 40 of 151 results