...supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ...

...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...

... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

...mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to ...

...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

...technologies become available almost daily, yet obstacles remain. One of the biggest challenges facing researchers...

...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

...from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores vision in...

...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...

...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

...types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers can see great...

...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...

...(10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing glucose and L-arabinose...

...your first grant, how you will overcome certain obstacles, alternative approaches, potential collaborations...

...Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of your narrative...

...clonal heterogeneity may represent a more serious obstacle to the generation of truly isogenic cell lines...

...incubate the membranes overnight in 500 mM to 1 M NaCl in order to enhance folding of the recombinant proteins...

...professional internationalization faces several obstacles; for instance there are few resources to help ...

... where getting hired hinges on performing near miracles. When evidence does not affirm our perception ...

...Pluronic-F68 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer...in PBS + 200 mM NaCl (C) (formulation buffer) Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure...impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules... buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of ...: mix 4.5 mL of 60% iodixanol and 13.5 mL of 1 M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60%... A C: 0.001% in PBS: 45 mL PBS + 5 ml B + 200 mM NaCl Procedure Cover the filter membrane with 15 mL of...through. Add 15 mL of 0.001% Pluronic F68 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min at 4 °C and discard...