We narrowed to 46 results for: ACL

...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...

... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

...mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to ...

...technologies become available almost daily, yet obstacles remain. One of the biggest challenges facing researchers...

...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

...from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores vision in...

...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...

...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

...(10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing glucose and L-arabinose...

...types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers can see great...

...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...

...your first grant, how you will overcome certain obstacles, alternative approaches, potential collaborations...

...Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of your narrative...

...incubate the membranes overnight in 500 mM to 1 M NaCl in order to enhance folding of the recombinant proteins...

...professional internationalization faces several obstacles; for instance there are few resources to help ...

...clonal heterogeneity may represent a more serious obstacle to the generation of truly isogenic cell lines...

... where getting hired hinges on performing near miracles. When evidence does not affirm our perception ...

...Poloxamer 188 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer...in PBS + 200 mM NaCl (C) (formulation buffer) Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure...impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules... buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of ...: mix 4.5 mL of 60% iodixanol and 13.5 mL of 1 M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60%... A C: 0.001% in PBS: 45 mL PBS + 5 ml B + 200 mM NaCl Procedure Cover the filter membrane with 15 mL of...through. Add 15 mL of 0.001% Poloxamer 188 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min at 4 °C and discard...

...Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation...solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl into deionized water and adjust to a final volume... °C. Cell Lysis Buffer : 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile...