Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 46 results for: ACL

Showing: 21 - 40 of 46 results

  3. PhD Applications After COVID

    Type
    Blog Post
    ...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores vision in...

  19. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Pluronic-F68 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer...in PBS + 200 mM NaCl (C) (formulation buffer) Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure...impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules... buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of ...: mix 4.5 mL of 60% iodixanol and 13.5 mL of 1 M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60%... A C: 0.001% in PBS: 45 mL PBS + 5 ml B + 200 mM NaCl Procedure Cover the filter membrane with 15 mL of...through. Add 15 mL of 0.001% Pluronic F68 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min at 4 °C and discard...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation...solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl into deionized water and adjust to a final volume... °C. Cell Lysis Buffer : 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile...
Showing: 21 - 40 of 46 results