We narrowed to 35 results for: BFP

1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ...Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed...fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). Some of the articles...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   ...of a miRNA target site. One fluorescent protein (EBFP2) is expressed in an unregulated fashion and thus...it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to define a ...

3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   ...fluorescent proteins for imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   ...luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope...

5. MXS Chaining

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   Blog Post

   ... Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean...

6. Hot Plasmids Spring 2024

   Type

   Blog Post

   ... by Puromycin, Zeocin®, or nuclear expression of BFP2.     Figure 1: CROPseq-multi uses two sgRNAs...

7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ...non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway...

8. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf Jaenisch’s lab, with ...

9. 15 Hot Plasmids from 2017

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   Blog Post

   ...nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and...

10. Fluorescent Protein Guide: Empty Backbones

    Type

    Collection

    ...Expression EBFP2 383 448 17 4.5 Prone to dimerization pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian...Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - Bacterial Expression moxBFP 385 448 17 Monomer...Sirius-N1 - Mammalian Expression SBFP2 380 446 13 Monomer (A206K) pSBFP2-C1 - Mammalian Expression Azurite...Bacterial Expression mTagBFP2 399 456 32 2.7 12 min Prone to dimerization mTagBFP2-pBAD - Bacterial Expression...-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression mAzurite 383 450 14...Monomer (A206K) moxBFP - Mammalian Expression mKalama1 385 456 16 5.5 Monomer (A206K) pBad-mKalama1 - Bacterial...

11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type

    Collection

    ..., T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-terminal mTagBFP2 tag pNIC-GST-TEV-...plasmid expressing mCherry pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...

12. Recombinases AAV Preps

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    Collection

    ...AAV-pgk-Cre PGK none rg* Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40...

13. Brain Initiative Collection

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    Collection

    ...with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for...

14. AAV for Neuronal Tracing

    Type

    Collection

    ...rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to Rabies Virus-based...

15. Tetracycline Inducible Expression

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    Collection

    ...monosynaptic tracing. To be coinjected with pAAV-TREtight-mTagBFP2-B19G ( Plasmid #100799 ). Ian Wickersham 172878...