We narrowed to 114 results for: CHL
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TypeBlog Post...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...
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The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...post. References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45... not what most readers want to read. As Susanna Bachle points out in her talk (29:31 - 49:45), there are... -
Plant Plasmids and Resources
TypeCollection...Stuttmann. MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons for chloroplast transformation... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current standard version known... (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...for phototaxis in the unicellular eukaryote Chlamydomonas." Nature 311.5988 (1984): 756. PubMed PMID: ...Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991): 489. Hirsch, Matthew... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...yellow-light sensitive chloride ion pumps, activation of which results in efflux of chloride ions, subsequent... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...or commercial workbenches such as MacVector or Benchling. Utility functions: These functions help speed... so-called genome editing workbenches such as Benchling (commercial, online), DESKGEN (commercial, online... -
Biosensor AAV Preps
TypeCollection...none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors iGABASnFR iGABASnFR2 Glutamate... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...agents as guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure... -
Brain Initiative Collection
TypeCollection...AAV9 pAAV_hSynapsin_psychLight2 To generate Adeno-Associated viruses for expression of psychLight2 under... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini... -
Microbes: Look, Listen, and Tell
TypeBlog Post... Susanna Bachle... -
CRISPR Guide
TypeCollection...alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia..., O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., Van Der Oost, ... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...By Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post... Susanna Bachle... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...but when I met the astronaut Jim Lovell I was speechless (the club of people who have been in space is...