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We narrowed to 253 results for: CIL

Showing: 21 - 40 of 253 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1...pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin. Carbenicillin is more stable, so ...Working Concentration Ampicillin 100 mg/mL 100 µg/mL Bleocin 5 mg/mL 5 µg/mL Carbenicillin* 100 mg/mL 100 µg...plates with a final concentration of 100 ug/mL ampicillin, you should make a stock solution of 100,000 .../mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it to 1 mL of water, dissolve by ...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ..., cells with a penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying ...neighbors is to eliminate the competition, and Penicillium isn’t the only organism that knows it. Some bacteria...whole-genome sequencing becomes more relevant (Garcillán-Barcia et al., 2023). In this post, we’ve grouped...e16. https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...excision repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (...relies on the efficient delivery of donor DNA to facilitate repair. Since the development of CRISPR-mediated...the CBE deaminates a target cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either...cellular inosine excision repair is not as active as uracil excision, ABE does not require any additional inhibitor...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... They are present in most of the cell culture facilities, tissue culture labs and every cell culturist...resistant to antibiotics and antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through... and guidance to the people working in the lab facility. This can be done by training eligible staff for...lines. Providing such an environment for the lab facility will not only reduce mycoplasmas but also avoid...Equipment Fumigate the laminar airflow and the lab facility periodically. The CO2 incubators, used for maintaining...

  6. Brain Initiative Collection

    Type
    Collection
    ...Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced monoclonal antibodies...Service . Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...These fusions convert cytosine to uracil without cutting DNA. Uracil is then subsequently converted to...DNA replication or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision... without producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. ...These byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair...

  8. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids... nucleotides (for example, Digoxigenin-labelled Uracil) that will bind to complementary mRNA within an...designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These...the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... or C terminal of a protein and can inducibly facilitate the degradation of the tagged protein. Degrons...here are all conditional, meaning that they only facilitate degradation of their fused protein under certain...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the degron tag with...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...also use different cytoskeletal machineries to facilitate transport: retrograde transport relies on dynein...2014). It is currently unclear which proteins facilitate transport of neuronal tracing compounds and this...-Gold (Schmued and Fallon, 1986) are used. To facilitate cellular uptake of these dyes in vivo, microinjection...rAAV2-retro’s altered amino acid sequence might facilitate interaction with uptake or intracellular transport...mechanisms of intracellular transport of AAV could facilitate the development of more powerful AAV serotypes...

  11. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010...Gram-positive  Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A..., Streptomyces lividans, Synechococcus spp., Thiobacillus ferrooxidans, Vibrio salmonicida, Yersinia enterocolitica...Gram-negative  Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter...

  12. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...characteristics and capabilities an ELN needs to facilitate true reproducibility. The following are critical...making it easy to get data out of the ELN; and (2) facilitate inter-operability with other research tools and...describe how each of these kinds of connectivity facilitates reproducibility. Connectivity to other data sources...Framework, Data Dryad, and Figshare. Any ELN that facilitates reproducibility has to make it easy to export...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience...Protocols References The UC Davis/NIH NeuroMab Facility (Link opens in a new window) together with the...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful...

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast, mammalian...His, Avi, and GFP) as well as protease sites to facilitate protein purification. We hope this kit will allow...Furthermore, some plasmids contain peptides to facilitate protein folding and/or protease sites to remove...kit can be used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your...

  15. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...mutagenesis generally applies to plasmids, but may also facilitate genome editing. Tailored mutations are commonly...high homology to the endogenous target, yet to facilitate the repair, the template should be resistant ... restriction site through mutagenesis vastly facilitates the subsequent process of screening for succesfully...template. Amplification of GC-rich plasmids is facilitated by the addition of DMSO to the PCR reaction (...

  16. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ...results you get back from your favorite sequencing facility. When it comes to DNA sequencing the chromatogram...your sample or consider asking your sequencing facility to apply a special protocol for a difficult template...you send for DNA sequencing If your sequencing facility requires you to perform your own Big Dye PCR amplification...

  17. Cre-lox system

    Type
    Collection
    ...higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven recombination. CREM... knocked in to a mouse at the Rosa26 locus to facilitate Cas9 mediated genome editing in multiple tissues...

  18. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...devised means of co-opting phage genetic systems to facilitate homologous recombination in bacteria. These quirks...of just a single organism, such as Clostridium difficile, could be targeted and preferentially depleted...system could be further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on...DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials...

  19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

  20. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...are used in the growth media. ura4+ pyrimidine (uracil) yes - Grow with 5-FOA*. S. pombe  no no *FOA in...
Showing: 21 - 40 of 253 results