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Addgene

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We narrowed to 103 results for: EXO

Showing: 21 - 40 of 103 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...popular for the modulation of endogenously and exogenously expressed proteins. Here we will review what ...certain conditions (typically the addition of an exogenous small molecule). Degron tags can range in size...tags can be used to manipulate endogenously and exogenously expressed proteins. Thirdly, degrons and their...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... fall into two categories: those that rely on exogenous enzymes and those that use endogenous homologous...recombination. Modifying bacterial genomes using exogenous enzymes: Lambda red and CRISPR Developed over ... these systems are powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...thermophilus to predatory phages to test if the exogenous DNA would be incorporated into the bacterial genome..., which guide a Cas nuclease to the specified exogenous genetic material next to the species-specific ...

  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...inserted a linker and GFP tag at the end of the last exon of the gene to ensure it would be transcribed and...inserting the tag and the linker preceding the first exon of the gene. In this manner, we have created an ...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...), 3’->5’ exonuclease activity (increases amplification fidelity), and no 5’->3’ exonuclease activity ...

  14. Ras Pathway

    Type
    Collection
    ...transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4... Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612 Anti-Kv2.1...Human Mouse IgG2a 182108 Anti-TRIP8b (exon 1b) [N212A/34R] TRIP8b (exon 1b) Mouse Mouse IgG2a 182109 Anti-...CLN3 Human Mouse IgG2a 206565 Anti-MFF (exon 1) [N382/69R] MFF (exon 1) Human Mouse IgG2a 206566 Anti-Ankyrin-R...225389 TRIP8b (exon 1a-5) (Mus musculus) recombinant monoclonal antibody. TRIP8b (exon 1a-5) Mouse Mouse... 225413 TRIP8b (exon 1b) (Mus musculus) recombinant monoclonal antibody. TRIP8b (exon 1b) Mouse Mouse ...Mouse Mouse 190538 TRIP8b (exon 1b) scFv [N212A/34] N212A/34 scFv TRIP8b (exon 1b) Mouse Mouse 190539 Shank1...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human CRISPR Poison Exon Knockout Library 138084 Knockout Human Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR...Knockout Human Wei 3rd Varies 12,472 pairs HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human...Human Stiewe N/A Varies 1,910–3,034 per exon Human Cas13 lncRNA Library 227014 RNA Knockdown Human Sanjana...Human Moffat Blencowe 3rd variable 92,746 CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout Human...

  17. Plan Your Experiment

    Type
    Collection
    ...Cas9 nickase) Single (or dual) gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes...gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances that the targeted region...removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since frameshift... Alternatively, gRNAs can be designed to target exons that code for known essential protein domains. The...
Showing: 21 - 40 of 103 results