We narrowed to 56 results for: Flp
-
TypeCollection...fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2...mCherry Flp dependent 1 Connie Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a ChR2/H134R EYFP Flp dependent... Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Edward Boyden...pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Karl Deisseroth 137143 pAAV-nEF-Con/Foff... pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth 137163 pAAV-nEF-Con/Foff...Fon hChR2(H134R)-EYFP Syn ChR2/H134R EYFP Cre and Flp dependent 5, 8, 9, rg* Karl Deisseroth 58880 pAAV-Syn-ChR2...Fon-ChR2(ET/TC)-EYFP nEF ChR2(E123T/T159C) EYFP Cre and Flp dependent 8 Karl Deisseroth 137140 pAAV-nEF-Con/Foff...
-
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634 AAV1 pAAV-EF1a-mCherry-IRES-Flpo 55637 AAV1 pAAV-EF1a-Flpo Calcium... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff/Fon-sRGECO ... -
Biosensor AAV Preps
TypeCollection...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Flp dependent 8 Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a GCaMP6m none Cre, Flp and VCre... 128315 pAAV-Ef1a-fDIO-GCaMP6f EF1a GCaMP6f none Flp dependent 1 Jensen 135632 pAAV-S5E2-GCaMP6f E2 GCaMP6f...pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f none Cre and Flp dependent 5, 8 Deisseroth 137123 pAAV-Ef1a-Con/Foff...137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2...pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff ... 105714 pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s... -
Retrograde AAV viral preps
TypeCollection...Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m ...Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Karl Deisseroth 28306 pAAV-FLEX-tdTomato... Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth 114470 pAAV-Ef1a-mCherry...Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth 99130 pAAV-mDlx-NLS-mRuby2... 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP, Cre, Flp and VCre-dependent Control Karl Deisseroth 117382...Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases Esteban Engel , Alexander...Con/Fon hChR2(H134R)-EYFP Syn Activator, Cre and Flp-dependent Optogenetics Karl Deisseroth 58880 pAAV-Syn-ChR2... -
Chemogenetics AAV Preps
TypeCollection...mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent...mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent 8, rg* Ulrik Gether 154868 pAAV-hSyn-fDIO-hM3D...mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* Ulrik Gether 119741 AAV SYN flex...Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre and Flp-dependent 8 Karl Deisseroth 177673 pAAV-nEF-Con/Foff... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection pathway neurons and ... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview: Ed Boyden... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...-NES-OCaMP-WPRE pAAV hSyn pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE pAAV Ef1a pAAV-hSyn-FLEX(cre)... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...EF1 promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP...72260 Expresses copGFP from the EF1 promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato...expressing a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer...from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance...resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter...tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe and tdTomato from the EF1 promoter... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...now available in a ready to use AAV5 viral prep. Flp-dependent DREADDs from the Gether lab, now available... -
Worm Expression Resources
TypeCollection...diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis...expression in C. elegans by combining expression of the FLP recombinase from either a heat-shock promoter or ...tissue-specific promoter and expression of the target FLP-out cassette from either a ubiquitous or a tissue-specific... -
Rabies and Neuronal Tracing
TypeBlog Post... to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...approach proved a challenge. The old standby of CRE/flp didn’t work – it turns out these tyrosine recombinases... -
CRISPR Plasmids - Tagging
TypeCollection.... An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out. Plasmids...assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and... -
AAV Molecular Tools
TypeCollection...pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent expression of the voltage-gated ... -
AAV Viral Preps
TypeCollection...Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)... -
Plasmids 101: Repressible Promoters
TypeBlog Post...split GAL4 architecture or combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al. ...