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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 395 results for: ICL

Showing: 21 - 40 of 395 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...Lentiviral Tet-On vector for inducible expression. See article ( Meerbrey et al., 2011 ) for additional tookit...transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional selection...inducible expression of transgene and/or shRNA. See article ( Szulc et al., 2006 ) for Tet-Off and additional...vector, expresses tTA from the CAG promoter. See article ( Chan et al., 2017 ) for other viral tTA plasmids...

  2. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...which enable the production of infectious AAV particles without the use of a helper virus. Instead, specific...produce AAV. In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A...Addgene) may be required to produce infectious AAV particles. You can search or sort this table based on plasmid...

  3. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...publication link to view all other plasmids from that article. Click on the PI link to view all available plasmids...

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...about the repression system can be found in this article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard...chromosomal integration of pLC97 can be found in this article: St-Pierre, F., Cui, L., Priest, D. G., Endy, D...

  5. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Order a high-quality... examples of quality control data. Help Center Articles Additional details about viral vector production...

  6. Adenovirus Plasmids

    Type
    Collection
    ...mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide for more information...pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...generally represents the physical number of viral particles. Users may need to run lower or higher dilutions...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...filters made of materials other than PES. AAV particles stick to many other surfaces, but do not stick...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Featured article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell...download the full-text of the publication here . Article Citation Generation of Genomic Deletions in Mammalian...

  10. CRISPR Library Amplification

    Type
    Protocol
    ... are not efficiently packaged into lentiviral particles. See below for options if the recombinant band...

  11. Plan Your Experiment

    Type
    Collection
    ...produce the viral particles. Then you transduce your cells using the viral particles before you continue...

  12. Brain Initiative Collection

    Type
    Collection
    ...high-titer 100 µL solutions of purified viral particles that are suitable for in vivo use. Learn more ...AAV are distributed as 100 µL of purified viral particles in solution that are suitable for in vivo use....

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...saw that these AAV particles come in a slightly different shape. These AAV particles are often found with...with cellular exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are... found that the association of AAV particles with membrane vesicles protects them from antibody recognition...exosome AAVs is related to the use of microvesicles as a vehicle for drug delivery that has recently gained...During vector production, assembled AAV vector particles accumulate inside the producer cells and purification...process that begins with cell lysis to release AAV particles with subsequent purification steps. It’s also ...cellular membrane into the culture medium. AAV particles are either associated with the outside surface...

  14. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...getting these particles to infect cells is also very titer-dependent. In order for an AAV particle to infect...causing less particles to be released into suspension and lowering the number of viral particles that can ...generating viral particles. Limit tube transfer - the fewer opportunities your viral particles have to stick...contact with a cell Although an individual AAV particle’s ability to infect a cell is dependent on its ..., you can see the dwindling proportion of AAV particles that make it through each part of the cell on ...expressed. The bottom line is that a lot of AAV particles are needed to get a few good ones into a cell ...contact between the cell and any individual AAV particle is rare. But simply getting into the cell may ...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...by the ratio of physical viral particles to infectious viral particles. Ratio of full to empty viral capsids...vector particles. This image shows that the vast majority of the vectors consist of full particles (white...? Physical titer: the concentration of viral particles containing viral genomes. Physical titers are ...quantification methods - see below), since each viral particle typically contains one viral genome. Other than... Infectious titer: the concentration of viral particles that can transduce cells. Infectious titers are... to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner et al., 2010). However, ...the same study reported a physical-to-infectious particle ratio of 50:1 (Zeltner et al., 2010). The specific...

  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ... gas vesicles gene clusters from a diverse set of gas vesicle-expressing species. The gas vesicle genes...flos-aquae gas vesicle gene cluster because of its small size and its ability to produce gas vesicles with nonlinear...of air-filled protein nanostructures called gas vesicles, which scatter sound waves and thereby produce...pBAD-bARGSer-AxeTxe (Fig. 1a).  With pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells...arabinose-containing plates will be visibly white, and gas vesicles in cells are visible under both phase contrast...microscopy and electron microscopy (Fig. 1b). Gas vesicle expression in vivo is also straightforward: after... before imaging with ultrasound to observe gas vesicle-specific ultrasound contrast where the bacteria...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... be integrated into the new viral particles and the virus particles produced could not replicate. However...favourite cell line or into primary cells, this blog article will give you some tips to plan your experiment... genes necessary for the production of a viral particle (Gag, Pol, Tat, Rev and Env) and the transfer ...necessary to encapsulate that insert into a viral particle (the packaging signal Psi). Because the packaging...and broadens the cellular tropism of the viral particles produced. However, in this vector production system...essential for the production of the lentiviral particle have been removed. This generation is safer than...set-up and purify and concentrate your lentiviral particles to get them to the optimum concentration to infect...

  18. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...proteins that form bacterial gas vesicles. These ~200nm long vesicles have hollow interiors and a protein...and arg2 vesicles is the amount of soundwave pressure required to collapse them: arg2 vesicles collapse...collapse at a lower pressure than arg1 vesicles. Following collapse, vesicles are invisible to ultrasound, which...(ARGs) overcome these limitations by using gas vesicle reporters that are detectable by an inexpensive...that excludes water but is gas permeable. These vesicles are primarily used by water-dwelling photosynthetic.... The Shapiro lab recently discovered that gas vesicles also scatter sound waves and therefore produce...resolution. By piecing together genes from gas vesicle operons from two different bacteria, Bourdeau et...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...infectious titer, meaning the concentration of viral particles that can transduce cells, which can vary based...physical titers, meaning the concentration of viral particles containing a viral genome. While ddPCR is superior... sequences. Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089/hgtb.2011.034 Benyamini...in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186/s13024-020-00361-z Grimm...& Kleinschmidt, J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes...production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt.3300946 Howard... 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089/hgtb.2016.180 Korte,...

  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...of the vector particle, some of the RT protein gets packaged inside of the vector particle, along with ...you consider that out of the 6 x 106 virus-like particles that we come into contact with every day (Prussin...understanding of the structure of a wild-type viral particle — and of the normal, replicative life cycle — ...detailed view of the structure of a retroviral particle, which is essentially the same for viruses and...How it is, exactly, that heaps of viral vector particles are made while missing the polymerase gene may...envelope during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go...cycle” in order to understand what these vector particles do once they come in contact with target cells...
Showing: 21 - 40 of 395 results