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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 98 results for: MLS

Showing: 21 - 40 of 98 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ...20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a...

  2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...–701 . https://doi.org/10.1038/nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little...:6–8 . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio A (2015) Improved dual ...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...standard curve accordingly.    Figure 1: A 1 mg/mL control is diluted in a series ranging from 1:2 to...the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651 nm.     How “good” ...

  6. Viral Production at Addgene

    Type
    Blog Post
    ... ensured to be less than five endotoxin units per mL. The endotoxin levels are determined using a chromogenic...-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression...

  7. Molecular Biology Reference

    Type
    Guide
    ...) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL...Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH.../mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing ...example, to make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X...1000X stock) to 100 mL of LB. DNA sequencing for plasmid verification DNA is made up of 4 bases, adenine...

  10. Antibody Production

    Type
    Collection
    ...concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration is confirmed by Coomassie...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...filter paper and saturate each with lysis solution (~3 ml each), pouring off any excess. When your protein ...in log phase) in blocking solution (around 108 CFU/mL is a good place to start) and add your membranes ...

  16. p53 Pathway

    Type
    Collection
    ...CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...paper. AAV can be safely concentrated to ~1x1014 vg/ml.. As evident in our repository, this application ...
Showing: 21 - 40 of 98 results