...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting...Crystal's educational background and position at Novartis. Chapter 2: What's a lab group in industry? The...empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself for success by having... The types of science that Crystal works on at Novartis, how she's learned to prioritize projects, and...listings. Mobility between different positions at Novartis. Chapter 7: How Crystal developed her science ...communication skills and how they've helped her at Novartis. The importance of mentorship in developing career...

...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

... the annealed DNA fragments. Taq DNA Ligase - covalently joins the annealed complementary DNA fragments...

...✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation that may optionally...repair system, and more recent prime editing innovations have improved the odds of mismatch repair favoring... C-terminus of Cas9 H840A nickase (PE1). But innovations by the Liu lab and others have led to a variety...cells with 1-10% indels. Since then, further innovations have continued to improve the maximum efficiency...expression and activity In addition to the numerous innovations that streamline the process of prime editing,...reduced editing efficiencies, likely because both innovations aim to solve the same problem. Both approaches... this table helps you keep them straight! Innovation Purpose PE2 PE3 PE4 PE5 PEmax PE6...

...catalytic activity and instead become trapped in a covalent bond with its substrate. Then, all it takes is...on the human DNA repair protein AGT that gets covalently linked to derivatives of its substrate O6-benzylguanine... 2005). Although this tight interaction is not covalent, later work showed that introducing a Leu28Cys...Leu28Cys mutation in eDHFR does lead to covalent binding of the TMP-tag ligand (Gallagher et al., 2009). HaloTag...substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate...Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent TMP-Tag Based on Proximity-Induced Reactivity.... Adams, S. R., & Tsien, R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside...

...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

...However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable...ll delve into strategies you can use to foster innovation. If you identify as a non-creative or if you ... by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself a block of...As a result, the product team is an incredibly innovative group. Our entire company benefits from their... about the future will improve your ability to innovate. (Finkelstein, 2019) One issue currently plaguing... S. (2019, July 15). A brief exercise to spur innovation on your team. Harvard Business Review. Retrieved...https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-your-team   Additional Resources on the Addgene...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier...

...AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex,...Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to ...their most potent Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel...observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector...genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods...

... ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount of background...

... by our partners at the Institute for Protein Innovation (IPI), part of a new collection of epitope tag...Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). This construct contains the Lon...substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license. This construct...amenable to a wide range of biophysical assays (Kroupova et al., 2024). They were able to study and crystallize...degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of a Cereblon construct...

...Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander....054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

...ligase to covalently link the fragments together at the expense of ATP (see below, covalent bonds are ...restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby... to make bonds between the insert and backbone covalent.  Before beginning the restriction digest...orientation. You then add ligase to the mixture to covalently link the backbone and insert and, PRESTO, you...

...of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize a transposon...inactivated Tc1/mariner transposons found in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred...or negative selected for during the experiment (Sandoval-Villegas et al., 2021). Target site preference... including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons...topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021...

...mycoplasma removal from the infected culture. MRAs are non-toxic, effective in the removal of most of ...Started with Tissue Culture below! Mycoplasma removal after detection So you found mycoplasma contamination...contamination to other healthy cell lines. Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family...mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid of most of mycoplasma...

...our partnership with the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly...enter 2025, your feedback continues to guide our innovation.  In response to a demand for more AAV viral ...extensive plasmid database — because we believe that innovation flourishes when data are shared. The scientific...look ahead, I am excited to continue to grow and innovate with your help and guidance. Every plasmid shared...

...Collection The Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to...brain through the development and application of innovative tools enabling large-scale real-time interrogation...

...immobilization for kinetic analysis Proteolytic removal of the fusion protein could provide a more relevant...second immobilization method. One possibility is covalent modification of the purified target protein with...and neutravidin) is for all practical purposes covalent. Therefore, protein immobilization through biotin-avidin...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately...

...technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods...tagging of proteins with mAID and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes...potential of mining datasets for research and innovation. Find MerMAIDs plasmids at Addgene Opperman J...

...new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s ...