We narrowed to 231 results for: PID

...restriction enzyme site and unique primer sites for more rapid screening by restriction digest and Sanger sequencing... sites, this technique also allows for easy and rapid targeting of complex elements to specific genomic...genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing is...(ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations and combinatorial... al. J. Mol. Biol. 2015. PubMed PMID: 26264872 Rapid, conditional degradation of essential proteins using...for new actuators that are non-invasive and can rapidly and reversibly stimulate neurons. Recent studies...

...Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional....2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian...Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors...Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold...

...reason this research has been able to progress so rapidly is scientific sharing. Already we’ve seen articles...and data associated with COVID-19 research have rapidly been developed. We asked the individuals behind...is giving Rutgers investigators the agility to rapidly initiate complex research projects that they would...

... is relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...Studies on the kinetics of these sensors showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, ... genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish...

...the viral genome and proteins. This is, for the intrepid researcher looking for molecular biology tools...protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid, called an envelope...copies, which are released from the cell like baby spiders from an egg sac. (Okay, some enveloped viruses ...

...chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome replication more...types of fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before...T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL...

...levels? Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away...antibody targeting the protein of interest led to rapid, targeted degradation that is dependent on proteasome...FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...

... of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine...oxysterols, bile acids, and numerous xenobiotic lipids derived from the diet. Additional members of this...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866...

...receptors and transporters, are integrated into lipid bilayers. Fusion of an FP to a transmembrane protein...localized to the endoplasmic reticulum, the normally spiderweb-like pattern of tubules can be grossly distorted...PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...

...W. (1983). Worldwide epidemiology of human adenovirus infections. Am J Epidemiol, 117(4), 455–466. https...was first isolated from human adenoid tissue and rapidly caused the death of adenoid cells in vitro (Rowe...

...cell line. This design allows highly localized, rapid and reversible microtubule severing and has very... sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine...

...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...research project was hard; I was researching the epidemiology of breast cancer and I loved the practical aspects...would advise you to be wary of companies which are rapidly expanding, as this might not be sustainable. After...

...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....

...SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...

...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...

...respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream...Single fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...Single fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...sensors A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...

...Lck-mTurquoise2 Plasma membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I...Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-...pLCK-mScarlet3_C1 Plasma membrane Lck N-terminal lipidation signal mScarlet3 Dorus Gadella 61180 pCMU-PMr...

...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...advancing this field and how we are able to support rapid progress in all areas of research. We welcome your...

...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...