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We narrowed to 229 results for: PID

Showing: 21 - 40 of 229 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... is relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...Studies on the kinetics of these sensors showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, ... genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...the viral genome and proteins. This is, for the intrepid researcher looking for molecular biology tools...protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid, called an envelope...copies, which are released from the cell like baby spiders from an egg sac. (Okay, some enveloped viruses ...

  4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome replication more...types of fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before...T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL...

  5. Working with Nuclear Receptors

    Type
    Blog Post
    ... of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine...oxysterols, bile acids, and numerous xenobiotic lipids derived from the diet. Additional members of this...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in...of genetics and developmental biology. With its rapid development (embryogenesis is complete in ~3 days...MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer drivers in vivo. Biotagging...

  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...W. (1983). Worldwide epidemiology of human adenovirus infections. Am J Epidemiol, 117(4), 455–466. https...was first isolated from human adenoid tissue and rapidly caused the death of adenoid cells in vitro (Rowe...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...cell line. This design allows highly localized, rapid and reversible microtubule severing and has very... sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine...

  11. Finding Your Perfect Job After University

    Type
    Blog Post
    ...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...research project was hard; I was researching the epidemiology of breast cancer and I loved the practical aspects...would advise you to be wary of companies which are rapidly expanding, as this might not be sustainable. After...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....

  13. Deep Dive: qPCR

    Type
    Blog Post
    ...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...portfolio of biomolecular tools and emphasizes their rapid and broad dissemination to the research community...available to the scientific community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal... new window) An organization with a mission to rapidly develop therapeutics that will slow the progression...

  15. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...advancing this field and how we are able to support rapid progress in all areas of research. We welcome your...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...

  17. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...of each construct. This new tool kit allows the rapid generation of stable transgenic cell lines which..., et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Biotechnol...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... Knockout Mouse Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249...Library 117725 Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human...

  19. AAV Production in HEK293 Cells

    Type
    Protocol
    ...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...
Showing: 21 - 40 of 229 results