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We narrowed to 1,017 results for: PLE

Showing: 21 - 40 of 1017 results

  1. Viral Production

    Type
    Collection
    ... lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting...Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures...specific QC experiments were performed on your lot, please contact us . Full sequencing of the Viral Genome...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral... In these mice the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent...

  3. Arf GTPase Family

    Type
    Collection
    ...cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of...ARF GTPase family, information on each gene, and examples of plasmid types available for each gene. Click...gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids ...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375 181 Bacterial... and GEF family, information on each gene, and examples of plasmid types available for each gene. Click...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP) 55738 ...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight junctions VE-cadherin mApple* Michael Davidson 55165... that has a different emission wavelength. For example, if the marker protein is fused to EGFP, you may...with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria...Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection...Microtubules alpha-Tubulin mCherry Gia Voeltz 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    .... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found...visualize and track clones of cells over time. Examples of useful tools for studying cells that share ... Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish, ranging ...for studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...pEG545 - Worm Expression mMaple3 491 568 506 583 405 5.8 12 Monomer pmMaple3-CAM - Bacterial Expression...? Visit our Empty Backbones page or search our complete collection and filter by vector type, popularity...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...smURFP 642 670 32 3.2 39 min Prone to dimerization pLenti-smURFP - Mammalian Expression Return to top Near-Infrared...Monomer pmiRFP670-N1 - Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...targeting the PITCh donor plasmid. Note that the principle of the PITCh system is different from the conventional...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...nuclear and cytoplasmic proteins, which can then be depleted very rapidly after the addition of auxin to the...and can be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the...

  8. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual..., to order more than 2 µg of a single plasmid, multiple orders would need to be placed through our website...website. Every order will need to be completed independently including the check out and will require separate...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

  9. Rett Syndrome

    Type
    Collection
    ...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...your plasmids! To get started with your deposit, please click here ....

  10. Antibody Production

    Type
    Collection
    ...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed... webpage. To learn more about these antibodies, please visit the Institute for Protein Innovation Antibody...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry (ICC...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ... TetR with the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This...expression with flexibility for your experiments. For example, you could integrate a transactivator under a tissue-specific... as a dual vector design, but it is easier to implement and ensures that all transfected or transduced...induction, or amount of background expression. For example, only a relatively low level of doxycycline can... ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector... selection rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector...expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-...

  12. Lentivirus Plasmids

    Type
    Collection
    ...infectious viral particle (a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene...plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with...2009) for additional variants. Eric Campeau 39481 pLenti-puro 3rd CMV-driven expression of cDNA with puromycin...-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway backbones for additional variants...selection. Kevin Brindle and Duncan Jodrell 17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression with CMV...of mitochondrial mKeima. Richard Youle 119816 pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression...

  13. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...imaging techniques, including that it is easy to implement due to the simplicity of gRNA design, programmable...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...copies of labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

  14. AAV for Neuronal Tracing

    Type
    Collection
    ...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the viral glycoprotein...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  15. Plant Plasmids and Resources

    Type
    Collection
    ... seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...

  16. Ras Pathway

    Type
    Collection
    ... browse the gene list below. For proteins with multiple subunits or isoforms, individual links to each...available plasmids for the gene. For components with multiple subunits or isoforms, individual links to each...EXOC2 EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast... cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ...movement. More than ten million people worldwide are living with PD, mostly people over the age of 60. There...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...defined FRT site. Multiplexing: Orthogonal recombinase systems can be combined in multiple, independent recombination...interacts only with their own target sites (for example, Cre with lox sites, Flp with FRT sites, Dre with...preferred codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate...translocation of a gene of interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent ...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  20. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... 12 GC/mL for the AAV2 serotype, as titered by droplet digital PCR (ddPCR) . In some cases, we may expect... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...for the import of biological research materials. Please be aware that additional customs charges may be...
Showing: 21 - 40 of 1017 results