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We narrowed to 929 results for: POR

Showing: 21 - 40 of 929 results

  1. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    ...Genome Institute of Singapore. Chapter 3: Biology research and resources in Singapore. Chapter 4: Life as...Chew, a researcher at the Genome Institute of Singapore who recently started his own lab. We discuss some...Principal Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies ... singapore...

  2. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...Problems with FRET kinase reporters Difficult to Multiplex: FRET reporters are comprised of two fluorescent...phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded...between the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...single-cell kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex...alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a...multiplexed together. Challenging  to Design: FRET reporter activity depends on both the distance and the ...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...- or reward-related behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

  4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into...Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian...contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists...heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance, and sometimes...Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression...with a resolution of less than 100 µm, the ARG reporters could be successfully visualized in vivo using...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit...

  5. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...exist for all Addgene plasmids! In an effort to support standardized persistent identification of research...Research Resource identifiers (RRIDs) which are supported by SciCrunch. Other well known PID formats are... the journal’s specific author guidelines for reporting your materials. If you deposit a plasmid with ...plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats. Acknowledgments...

  6. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are...Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized ...panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate ...Image from Diessl et al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Neurotransmitters Cell Physiology Browse All Other Reporters Additional Resources Fluorescent biosensors are...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

  9. Genetic Code Expansion

    Type
    Collection
    ...tRNA is incorporated to the growing peptide chain. To expand the genetic code and incorporate a ncAA into...by reassigning specific codons to enable the incorporation of amino acids beyond the 20 standard ones, ... cell, the ncAA provided in the media can be incorporated using the existing protein translation machinery... tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid often carries multiple tRNA...target plasmids in genome code expansion for the incorporation of non-canonical amino acids (ncAA) in a protein... are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...questions there. This peer-to-peer GCE community support service was set up in 2022 by the NIH-funded GCE4All...

  10. Bacterial Expression Systems

    Type
    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...

  11. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...purpose-driven nonprofit, Addgene offers expert support, educational resources, and efficient material ... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"...includes specialized Promega technologies that support a range of applications. The technology includes... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C...Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI CACNA1A GFP U6, Cbh Spinocerebellar...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1...

  13. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...Plasmid Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

  14. CRISPR References and Information

    Type
    Collection
    ...gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes...Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in a new window) This tool helps design...(2016) (Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral...input sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms...regulation (repression and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( ... or a sequence text file you upload. Currently supports a wide selection of organisms. From the Database...sequence and checks for off-target binding. Currently supports a wide range of organims. Developed by the Michael...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more... the expression cassette of interest is incorporated into the host DNA. For mammalian cells, you ...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... has developed several tools useful for the incorporation of phosphoserine into your gene of interest.... where it recognizes the UAG amber codon and incorporates phosphoserine into the growing polypeptide chain...University changed the way researchers can explore important questions surrounding serine phosphorylation by... of the popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain...

  17. Plan Your Experiment

    Type
    Collection
    ...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...generally perform by chemical transfection or electroporation. Expression can be transient, or you can generate... to target cells through microinjection or electroporation. The target cells translate the mRNA encoding...delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA... breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base and prime...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...genomic instability and cell death. It is important to incorporate appropriate controls such as Cre-only ...Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing...

  19. Brain Initiative Collection

    Type
    Collection
    ...disorders. The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...and antibodies created with support from the BRAIN Initiative below. Support has been identified by depositors...The BRAIN Initiative supports the development of biomolecular tools for neuroscience research. Browse...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal...128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin ...

  20. CRISPR Guide

    Type
    Collection
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...Overview of HDR. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the...
Showing: 21 - 40 of 929 results