Working with Nuclear Receptors
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Blog Post
...different tissues in normal and disease states, their circadian regulation, as well as in important physiological...
We narrowed to 44 results for: RCA
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Viral Vectors 101: Calcium Sensors
TypeBlog Post...insensitive. Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...Olfaction, temperature, humidity, noise level, circadian effects (inactive versus active phase) Experimenter... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks... -
Plasmids 101: Protein Expression
TypeBlog Post...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...addition of a probe based fluorescent dye that intercalates with any dsDNA and the use of a fluorometer ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...all double-stranded DNA, while Taqman uses an intercalating dye, with a fluorescent probe and quencher to... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A.... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...content. What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post... of antibiotics, which induce cell death by intercalating into DNA and generating DNA breaks. The Sh ble... -
An Introduction to Adenovirus
TypeBlog Post...between early and late gene transcription) is demarcated by viral genome replication (Harrach, 2014). ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...biology videos are: “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...current technologies Tetbow (Sakaguchi et al., 2018) overcame the low expression of XFP of Brainbow by incorporating... -
10 Steps to a Perfect Science Talk
TypeBlog Post...next?” Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of ... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...NA Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...22 to join in the March for Science. Cold and overcast with intermittent rains, it was weather befitting... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...A., and Kent Redman. "British anti-Lewisite (dimercaprol): an amazing history." Annals of emergency medicine... -
CRISPR Guide
TypeCollection...size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased ... Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9 system with high editing efficiency...