...Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene from the packaging...therefore recommended to test for the presence of RCA in the viral stock by doing a plaque formation assay...are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning...capacity, decreased  potential for the generation of RCA and reduced immunogenicity. In addition to E1/E3,...

...al., 2019. Looking for an alternative to intercalating fluorescent dyes for DNA-protein interaction...tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause photo-induced...

... start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

...Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E... favorite gene! SWI/SNF Genes:       BRG1(SMARCA4) BAF170 (SMARCC2) ARID1A   Chromatin research...

...components: the pigments (anthocyanins), and the plant circadian clock. Petunias have been a model organism for...characterized mechanisms and components of the circadian rhythm in petunias. Because of the extensive research...

...enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the... the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...

..., D., McDowell, I., Southern, K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards...., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A. M., Healy, L. M., Robertson...

...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...

...contains and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid...

...example, let’s say you identified gene target X for BRCA-deficient breast cancer and identified several candidate...

...: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by ...

...aggregate or precipitate out as insoluble granules. We overcame this problem by engineering a plasmid which contained...

...different tissues in normal and disease states, their circadian regulation, as well as in important physiological...

...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

...Olfaction, temperature, humidity, noise level, circadian effects (inactive versus active phase) Experimenter...

...insensitive. Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection...

...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for...

...all double-stranded DNA, while Taqman uses an intercalating dye, with a fluorescent probe and quencher to...

...addition of a probe based fluorescent dye that intercalates with any dsDNA and the use of a fluorometer ...

...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...