We narrowed to 33 results for: RCA

...Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene from the packaging...therefore recommended to test for the presence of RCA in the viral stock by doing a plaque formation assay...are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning...capacity, decreased  potential for the generation of RCA and reduced immunogenicity. In addition to E1/E3,...

...fluorescent and optogenetic tools. FRCaMPi uses an inverted topology of FRCaMP (itself based on mApple, which...Although similar in brightness to jRGECO1a and FRCaMP, FRCaMPi had better dynamic range and sensitivity, in...regions of live zebrafish larvae expressing FRCaMPi or SomaFRCaMPi in neurons; scale bars 50 µm. Reproduced...transfer tracker Red calcium indicators PinkyCaMP and FRCaMPi Micropeptide killswitch for biomolecular condensates....0510511103      Red calcium indicators PinkyCaMP and FRCaMPi By Mike Lacy Many popular genetically encoded calcium...Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from the Kiryl Piatkevich Lab (Zhou, Zhu, Eom, ...PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP had better brightness, ...

...al., 2019. Looking for an alternative to intercalating fluorescent dyes for DNA-protein interaction...tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause photo-induced...

... start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

...Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E... favorite gene! SWI/SNF Genes:       BRG1(SMARCA4) BAF170 (SMARCC2) ARID1A   Chromatin research...

...components: the pigments (anthocyanins), and the plant circadian clock. Petunias have been a model organism for...characterized mechanisms and components of the circadian rhythm in petunias. Because of the extensive research...

...enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the... the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...

..., D., McDowell, I., Southern, K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards...., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A. M., Healy, L. M., Robertson...

...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...

...contains and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid...

...example, let’s say you identified gene target X for BRCA-deficient breast cancer and identified several candidate...

...: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by ...

...fluorescence, while red indicators jRGECO1a and jRCaMP1a are widely used as well. To complement these tools...

...aggregate or precipitate out as insoluble granules. We overcame this problem by engineering a plasmid which contained...

...different tissues in normal and disease states, their circadian regulation, as well as in important physiological...

...Olfaction, temperature, humidity, noise level, circadian effects (inactive versus active phase) Experimenter...

...insensitive. Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection...

...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for...

...all double-stranded DNA, while Taqman uses an intercalating dye, with a fluorescent probe and quencher to...