We narrowed to 1,036 results for: REN

...codon usage biases of different organisms. Different species have different preferences for the codons they...scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis. They published...the gene may not express well because of these different codon usage biases. But if a gene’s sequence was...could be determined through comparisons with a reference sequence database. Through a series of simulations...Kunjapur says. Percent sequence identity was a different story - the team found that the percent sequence...sequences in Addgene’s database. They showed a growing trend of sourcing genes from distantly related organisms...and synthetic biology is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and...

...Koeris' own transition from graduate student to entrepreneur, listen to our full interview with Dr. Koeris...contrasts being a graduate student with being an entrepreneur 29:00 - 32:20 : What did It mean for Dr. Koeris...developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more important to develop something that...Board and how he hopes to help young biotech entrepreneurs.   We'd like to thank Dr. Koeris for taking... entrepreneur...

.... I spoke with Dr. Mike Koeris, co-founder and current VP of Business Development & Operations of Sample6...roles and skills necessary to be a succesful entrepreneur? As a co-founder of a biotech startup, Mike ...its pitch the best, wins. He explained that entrepreneurs must clearly communicate their idea at various...be reproducible by someone else. Second, "an entrepreneur must be OK with the constant fear of failure...are only successful if they are 100X better than current technology and get customers to change behavior...

...supporting in-person conference attendance, the award now supports virtual conference attendance. We also...Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners! ...awards help early career researchers attend a conference of their choice while honoring the late Michael...faster and more sensitive than its predecessors. Currently, Aggarwal, and colleagues Filip Tomaska and Tim...CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi Qiu.   He Tian: Directed...had improved performance in live mouse brains. Currently, she is using the improved voltage indicator coming...

...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...other and what to do.  Saved by the Tweet - The conference hashtag I had, however, started a Twitter account...at an institution where training opportunities weren’t always available. Even more valuable, however, ...like anyone at a meeting who felt isolated at a conference for any reason was a Singleton. Figure ... gene transfer, which occurs when bacteria of different evolutionary lineages share genes that can improve...

...proliferates and is redifferentiated to a different cell type. Transdifferentiation In this process, differentiated...reversal of a differentiated cell type to an undifferentiated state and then redifferentiation into the cell...variety of plasmids for differentiation here. Transdifferentiation Dedifferentiation to an intermediate pluripotent...ability to convert a differentiated cell to a different cell type. While transdifferentiation is suited for ... are converted to cells of a different lineage, without dedifferentiating into a pluripotent stage. Cellular...pluripotent state. Redifferentiation - Conversion of the pluripotent cells into differentiated cells of choice...control the cell fate of iPSCs and differentiate them into different cell lineages [13]. Over the last ...

...74444 pLenti.PGK.blast-Renilla_Luciferase Renilla PGK Lentiviral expression of Renilla luciferase Reuben Shaw...Root 118016 pLX313-Renilla luciferase Renilla EF1α Lentiviral expression of Renilla luciferase William...pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector...Orzaez 68215 pEGB 35S:Renilla:Tnos (GB0109) Renilla 35S Transcriptional unit for Renilla luciferase expression...depending on the application, and different luciferases require different substrates and detection wavelengths... than Renilla luciferase, but is more susceptible to enzyme inhibition. Both firefly and Renilla luciferase...12179 pIS1 Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion...

...Their ability to self-renew or replicate while maintaining the undifferentiated “stem” state and, Their...to self-renew is greatly diminished. Embryonic and adult stem cells can be used for different research...contributed by Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press reviews...special types of cells that can develop or “differentiate” into more specialized cells with specific functions...function adequately [1]. Stem cells’ ability to differentiate into multiple cell types makes them useful models...Their potency or capability to differentiate into specialized cell types [2]. We general speak of 4 levels... cell has the ability to produce all of the differentiated cells in an organism, including the embryonic...

...Constitutive 1 Loren Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Loren Looger 121922..., rg* Loren Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Loren Looger...Constitutive 1, 9 Loren Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Loren Looger..., rg* Loren Looger 176755 AAV-mDlx-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9, rg* Loren Looger...Constitutive 1, 9 Loren Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg* Loren Looger..., rg* Loren Looger 176754 AAV-mDlx-jGCaMP8m-WPRE Dlx jGCaMP8m none Constitutive 1, 9, rg* Loren Looger... 5, 9 Loren Looger 176757 AAV-EF1a-jGCaMP8m-WPRE EF1a jGCaMP8m none Constitutive 1, 9, rg* Loren Looger...

...Research Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids...unspecialized cells with the capacity to differentiate into many different cell types in the body during development...can be directly differentiated into specific somatic stem cells or fully differentiated cell types. Browse...collection of Differentiation Plasmids below. Alternatively, it is also possible to directly differentiate one ...one differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion... of Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and... , SOX2 , MYC , KLF4 , LIN28 . Differentiation and Transdifferentiation Plasmids Browse the table below...

...comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene...largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the video...community. Not surprising, this is where most of our conference room names originated from. Let’s take a look...Groucho, a transcriptional co-repressor The first conference room, that is actually used as a break room for...diffuses down a concentration gradient exerting a different effect on the developing embryo depending on its...required for spermatogenesis One of our smaller conference rooms is named Cookie Monster. cookie monster... bagpipe, two genes important for mesoderm differentiation Now, let's turn tinman and bagpipe, two genes...

...many different Cas proteins available, and researchers can adapt these experiments for different organisms...experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic manipulations...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S...have expanded the CRISPR toolbox to include many different types of edits, including point mutations, base...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will...Base editor (dCas9 or Cas9 nickase fusions with different base editing proteins) Many base editors fused...

...Biosensor Loren Looger AV-1-PV2724 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-5-PV2724 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-9-PV2724 98931-AAV9 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger ....2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509 98925-AAV1 pAAV.CAG.Ruby2sm-Flag.WPRE.SV40 Loren Looger AV-1-PV3854... list of Addgene's viral vectors See Addgene's Current AAV inventory , which includes all viral vectors...AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40...

...heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and ...of colors activated by different wavelengths, allowing for easy differentiation between multiple antibodies...roughly fifty different fluorophore colors in flow cytometry, but the largest panels used currently are in the...Antibodies are typically produced in one of three different methods: in animals, in cultured immune cells,...contain two Fab fragments, each recognizing a different epitope, bound by short peptide linkers. Diabodies...used as an antimicrobial agent. Other chemical interferences with conjugating reactions are possible. It ...chemiluminescent reaction in the presence of several different substrates. The luminescence produced by the reaction...

... CAG GFP Control Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG tdTomato Control Loren Looger 24593 AAV-pgk-Cre...Calcium sensor Loren Looger 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn H2B-GCaMP8s Calcium sensor Loren Looger 176750...Calcium sensor Loren Looger 176751 AAV-CamKIIa-jGCaMP8m-WPRE CamKII GCaMP8m Calcium sensor Loren Looger 176752...Calcium sensor Loren Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx GCaMP8f Calcium sensor Loren Looger 176755...Calcium sensor Loren Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium sensor Loren Looger 176757...Calcium sensor Loren Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium sensor Loren Looger 179254...Calcium sensor Loren Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium sensor Loren Looger 179256...

...created using BioRender.com (Link opens in a new window) . Addgene Website & Blog References Website CRISPR..., A., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell... into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they...pyrimidine-to-pyrimidine) Base transversions — edit to different classification of nucleotide base (purine-to-pyrimidine...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...

...information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal and adult...approximately 1:10,000. It is characterized by apparently normal development through the first 6 months... skills occurs. Diagnosis of Rett syndrome is currently based on specific clinical diagnostic criteria... known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W, R133C,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...

...PAQR2 ADM adrenomedullin AM ADM2 adrenomedullin 2 AM2, FLJ21135, dJ579N16.4 ADRB1 adrenergic, beta-1-,... MGC138246 GDF1 growth differentiation factor 1 - GDF10 growth differentiation factor 10 BMP-3b, BMP3B...BMP3B GDF11 growth differentiation factor 11 BMP-11, BMP11 GDF15 growth differentiation factor 15 GDF-15,... PTGFB GDF2 growth differentiation factor 2 BMP-9, BMP9 GDF3 growth differentiation factor 3 - GDF5 growth...growth differentiation factor 5 BMP14, CDMP1, LAP4, OS5, SYNS2 GDF6 growth differentiation factor 6 BMP13...MGC158101, SGM1 GDF7 growth differentiation factor 7 BMP12 GDF9 growth differentiation factor 9 - GDNF glial...PPH1, T-ALK GDF1 growth differentiation factor 1 - GDF10 growth differentiation factor 10 BMP-3b, BMP3B...

...for a different analyte, a sensor for gene-specific activity, or for popular biosensors in different expression...the articles linked below include variants with different binding affinities or negative-control constructs...3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren Looger , GENIE Project Calcium GCaMP3 genetically...indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors Engineering ...integrators. Science. 2015 Feb 13;347(6223):755-60. Loren Looger , Eric Schreiter Calcium Intersectional viral...cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP in vascular smooth muscle...chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride...

...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...Library CRISPR page for more information. Full references are below. Return to top Cas9 SAM transcriptional...The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library...Swiech et al., Nature Biotechnology 2014). Full references are below. Available plasmids are described below...The vector can be digested using BsaI ( This is different from BbsI, which is used in the SpCas9 plasmid...with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin mouse can be purchased...This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs targeting...