We narrowed to 92 results for: SAB
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TypeBlog Post...James McFarland, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, and Joshua Dempster from The Cancer Data...Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine...
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Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection...Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018) Field-deployable viral diagnostics using... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp. pDonor carries a customizable...form of RE-cargo-LE (RE, right end; LE, left end) pTnsABC expresses the transposases TnsA, TnsB, and TnsC... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...party library updates. Third party libraries are reusable software components that can be incorporated into...common name. While we do our best to write clean, reusable code when working on a feature, sometimes we have... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...structural information may guide design, it is advisable to generate multiple constructs with different...close to native expression levels. However, it is advisable to first express the fusion protein from a plasmid-based... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...pipettes for multiple cell lines rather than using disposable ones or reusing gloves. The serum is also a source... ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure... -
Cancer Research Plasmids and Resources
TypeCollection...Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic...Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute (NCI) NCI... -
Lentivirus Plasmids
TypeCollection...can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can be used for...EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for CMV driven... -
Ligation Independent Cloning
TypeProtocol...volume with no additional water (<5 μl). It is advisable to set up multiple reactions by holding the vector... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection... Brightness pKa Maturation Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange... mKorange IX - Mammalian Expression mKusabira Orange-C1 (mKO) - Mammalian Expression mKO-pBAD - Bacterial... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...very clean DNA, so using a little more enzyme is advisable. Reactions are often performed with 0.2-0.5 µL... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian/Lentiviral...Mammalian/Lentiviral hU6 none S. pyogenes Puro Sabatini sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) 71485 Mammalian... -
CRISPR Guide
TypeCollection...them referred to as INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting... 661–667. PMID: 25961408 Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014). Genetic screens...Livny, J., Regev, A., Koonin, E. V., Hung, D. T., Sabeti, P. C., Collins, J. J., & Zhang, F. (2017). Nucleic... -
2018 #DeckTheLab Contest Winners
TypeBlog Post...happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ... -
Addgene's Website Update
TypeBlog Post...little bit faster to skim through. Users with disabilities will find Addgene’s website easier to navigate... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...waste that needs to be supported at all levels. Disposables are an accepted part of lab culture, but with... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...FP blog posts. References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to ... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...used to construct 20kb long inserts. The easily reusable MXS-building blocks are particularly effective... Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...). Is it true that this many genes are truly dispensable or are the current identification methods missing... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...