We narrowed to 90 results for: SAB

...number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable...form of RE-cargo-LE (RE, right end; LE, left end) pTnsABC expresses the transposases TnsA, TnsB, and TnsC...

...Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection...Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018) Field-deployable viral diagnostics using...

...party library updates. Third party libraries are reusable software components that can be incorporated into...common name. While we do our best to write clean, reusable code when working on a feature, sometimes we have...

...structural information may guide design, it is advisable to generate multiple constructs with different...close to native expression levels. However, it is advisable to first express the fusion protein from a plasmid-based...

...pipettes for multiple cell lines rather than using disposable ones or reusing gloves. The serum is also a source... ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure...

...Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic...Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute (NCI) NCI...

...resistance. Can be used for cDNA expression. David Sabatini 14883 FUGW 3rd hUbC-driven EGFP. Can be used for...Abu-Remaileh et al. (2017) for additional variants. David Sabatini 171123 pLVX-TetOne-Puro-GFP 3rd Dox-inducible ...

...volume with no additional water (<5 μl). It is advisable to set up multiple reactions by holding the vector...

...very clean DNA, so using a little more enzyme is advisable. Reactions are often performed with 0.2-0.5 µL...

...them referred to as INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting... 661–667. PMID: 25961408 Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014). Genetic screens...Livny, J., Regev, A., Koonin, E. V., Hung, D. T., Sabeti, P. C., Collins, J. J., & Zhang, F. (2017). Nucleic...

...happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ...

...little bit faster to skim through. Users with disabilities will find Addgene’s website easier to navigate...

...waste that needs to be supported at all levels. Disposables are an accepted part of lab culture, but with...

...FP blog posts.     References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to ...

...used to construct 20kb long inserts. The easily reusable MXS-building blocks are particularly effective... Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...

...). Is it true that this many genes are truly dispensable or are the current identification methods missing...

...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...

..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

... contributes to behavior or pathology, and by disabling neurons you can figure out what they are necessary...

... proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist...