We narrowed to 36 results for: SKIP

1. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   ... you ever need to remake the same virus, you can skip the first round of production, saving about a week...

2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ...statistical modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ... take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293 cell culture...

4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   ...engineered DNA-binding molecules even enables us to skip the step of knocking-in. In 2013, we showed that...

5. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   ...peptides are thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus...

6. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   ...put this in print, but I actually let my daughter skip school a couple of times. Be sure to add time with...

7. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   ...Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA duplexes bind...

8. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   ...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...

9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   ...a fair amount of time, and it may be tempting to skip some steps and get to experiments. However, by optimizing...

10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    ...maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon...

11. Protocol - How to Purify DNA from an Agarose Gel

    Type

    Protocol

    ...contaminating your sample. To accomplish this, it is best to skip lanes between samples and between the ladder and...

12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    ...complementary edits to opposite strands of the DNA and skip the cellular mismatch repair step (Anzalone et al...

13. Deep Dive: qPCR

    Type

    Blog Post

    ...so pick one and use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon...

14. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

    ...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...

15. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...

16. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ... to the appropriate well. Pro-Tip When possible, skip one lane before loading samples. Load 20 µL of each...