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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 29 results for: SKIP

Showing: 1 - 20 of 29 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon skipping strategies...forms of dystrophin can still be functional, exon skipping is a good option for DMD treatment. Clinical trials... trials have used oligonucleotide exon skipping (OEN) to remove mutated exons from the dystrophin transcript... to explore genome editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired... Could this approach work with dystrophin exon skipping? For such a therapy to be successful, multiple...downregulated in mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining... and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation in dystrophin...

  4. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...allow scientists to conduct experiments faster, skipping the preparation steps of production, purification...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... an antibody can’t be used or that step can be skipped. It has the flexibility of also being used for ...

  7. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...for Postdocs and New Faculty" downloadable book. Skip to Chapter 3 for tips on how to develop your role...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...producing single-guide RNAs – but it also allows you to skip purification steps and end-repair steps with well-designed...

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... expected module order. (It’s also why you can’t skip a module entirely, but instead will have to replace...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...ready-to-use lentiviral preparation from Addgene, you can skip the steps above! Cells are then transduced with ...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... use them for, shuttle vectors can help you hop, skip, and jump between all kingdoms of life. A bit of...
Showing: 1 - 20 of 29 results