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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36 results for: SKIP

Showing: 1 - 20 of 36 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon skipping strategies...forms of dystrophin can still be functional, exon skipping is a good option for DMD treatment. Clinical trials... trials have used oligonucleotide exon skipping (OEN) to remove mutated exons from the dystrophin transcript... to explore genome editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired... Could this approach work with dystrophin exon skipping? For such a therapy to be successful, multiple...downregulated in mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining... and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation in dystrophin...

  4. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...allow scientists to conduct experiments faster, skipping the preparation steps of production, purification...

  7. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... an antibody can’t be used or that step can be skipped. It has the flexibility of also being used for ...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...producing single-guide RNAs – but it also allows you to skip purification steps and end-repair steps with well-designed...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

  16. Protocol - Bacterial Transformation

    Type
    Protocol
    ...heat-shock Shorten or skip the outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth,...plasmid) you can save a lot of time by shortening or skipping many steps and will still get enough colonies ...
Showing: 1 - 20 of 36 results