...future work. She graciously took me for coffee and spent an hour with me talking about the tools I could ...rapidly. The knowledge the Singletons gained from spending time with a mentor, as well as having a cohort...participants regarding the activities and time mentors spend with Singletons in their bins. We also sought expertise...

...subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings...system An electronics system  Cells are first resuspended in a pressurized buffer called sheath fluid and...instrument clogs, ensure that samples are single cell suspensions. For cells that tend to aggregate, pass the sample...

...that scientists have amazing stories to share. I spend a lot of time telling scientists about Addgene’s...how to deposit and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations...

... can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges are...

...biorender.com   Both gradients can be informative, so spend some time thinking about which type of gradient ... your blot’s data, the more time you’ll want to spend optimizing it. Blots for quantification generally...antibodies or validate them yourself, so you don’t spend time troubleshooting an assay that’s fine with antibodies...

...validated gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine...

...GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...

...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and ...Cambridge and Bristol. If you’re a scientist who spends many hours at a microscope, you too can become ...

... plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...

...scientists accomplish their goals. Many nonprofits must spend a lot of resources continuously raising money from...self-sustaining. With this unique model, we are able to spend resources on maintaining quality and starting new...

... into your interests and will determine how you spend a good chuck of your mental energy and time. The...At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

...though derivatives have been adapted for growth in suspension. Of the many derivatives of HEK-293, the most...systems, such as yeast. HEKs can also grow in suspension, which allows for large scalability, and can ...

...letters after your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking...entrepreneur at 22. Start with your network You've spent your life making connections and finding mentors...

... I was having outside of the “ivory tower.” I’d spend the afternoon lovingly tending to the stem cells...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...

...come here, I need to spend time here, this is an incredible place.” Doench has spent most of his research...

...Initially, he spent the majority of his time in the lab.  As time went on and he was spending more time in...

... makers to keep things legible. It is faster to spend more prep time on an experiment than it is to rerun...when I was tired, stressed, or rushing. I would spend the time quietly running through the experiment ...

...least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C ...products on the market to choose from) on your resuspended RNA sample will help ensure DNA contamination...

... the Scientific Support team, much of my day is spent helping to resolve problems, but even so, I wasn...framework to the myriad challenges we encounter. As you spend time in a lab learning techniques and taking on ...

... years as a senior scientist at Addgene. Having spent that long in customer service, I've daydreamed about...utility in the least amount of time. Though I now spend far less time answering help emails and phone calls...