We narrowed to 402 results for: SUA

...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

...fluorescent biosensors. Subcellular Localization Visualize organelles and subcellular structures with well-characterized...

...teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses in a Malate Dehydrogenase...

...protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect when the...

...special functions that vary from purification, visualization, and functional studies. You can even customize...

...mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time. Examples ...

...AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital...

... affected family member. These familial cases, usually inherited in an autosomal dominant manner, are ...

...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

...protein into your expression vector allows you to visualize which cells have undergone recombination. Reporter...

...protein, such as GFP, which allows you to visualize the cellular localization of your protein...

...transcriptions and create heritable epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind...

... restriction enzymes for further customization Usually paired with a sequencing method Can’t provide specific...

...In other cases, your experiment might rely on visualizing particular cellular structures or organelles,...

... that has UV light, visualize your DNA fragments. The fragments of DNA are usually referred to as ‘bands...DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical.... Pro-Tip TBE can be used instead of TAE, labs usually use one or the other, but there is very little ... concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel...gel). EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Caution ...loading less DNA into the well. Another method for visualizing very short DNA fragments is polyacrylamide gel...

...digestion (usually 3-6bp). Restriction Site: Your chosen restriction site for cloning (usually 6-8bp). Hybridization...primer that binds to the sequence to be amplified (usually 18-21bp). When selecting restriction sites, you...the desired location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not...genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give specificity and to also be compatible... run the product on a gel. This allows you to visualize that your PCR product is the anticipated size ...

...enzyme is usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually 37 °C...requires 1 µg of DNA. The total reaction volume usually varies from 10-50 µL depending on application and...manufacturer's instructions for more details. To visualize the results of your digest, conduct gel electrophoresis...

...transformation efficiency. The lowest efficiency cells (usually the least expensive) are fine for transforming ...incubate in 37°C incubator. Mix 1 - 5 μl of DNA (usually 10 pg - 100 ng) into 20-50 μL of competent cells...into a 42°C water bath for 30-60 secs (45 secs is usually ideal, but this varies depending on the competent...

...developed visual barcodes for clonal multiplexing of cells in live imaging application. Each visual barcode... reduced error rate for long sequence knock-ins Visual barcodes for live cell clonal multiplexing Improving...2022. https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes for live cell clonal multiplexing by: ...different fluorescent proteins, and inserting each visual barcode into its own subclone, they were able to... using freeware.    Fig. 4: Images of 12 visual barcodes in use in A375 cell line. Image from Kaufman...allowing for up to 20-plex experiments with one visual barcode per cell and up to 200-plex with two barcodes... or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman, et al. 2022. Nature...

...(AAV), have been used for tracing and visualizing motor, visual, and auditory neuronal circuitry in vivo...away from the site of application and aid in visualization of the morphology of individual neurons, including...colorimetric product or are fluorescent. While dyes usually spread unidirectionally, there are some reports...that their diffusion across the synaptic cleft is usually severely impaired, and only few reports exist about...number of connections, or the “connectome”, to be visualized in a uniform and standardized way (Oh et al.,...An additional desirable property for network visualization would be the ability to spread trans-synaptically...attributes and their use has allowed for the visualization of single connection networks within the rodent...