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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 37 results for: T7

Showing: 21 - 37 of 37 results

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    ...The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...

  3. CRISPR Antimicrobials

    Type
    Blog Post
    ... synthetic phages, each based on the well-studied T7 phage, to target various types of bacteria. In mixed...

  4. Depositor Collections

    Type
    Collection
    ...MORF) Collection Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor...

  5. TALEN Expression Vectors

    Type
    Collection
    ... CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription of RNA. TALENs...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...firefly luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase ...luciferase. Renilla luciferase is expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid...

  9. Bacterial Expression Systems

    Type
    Collection
    ...(aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic Esposito...Escherichia coli Cheryl Arrowsmith 26094 pET28a-LIC T7-lacO Lactose/IPTG Escherichia coli Cheryl Arrowsmith...Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia coli Cheryl Arrowsmith...

  12. Sequencing Primers

    Type
    Guide
    ... primer T7 TAATACGACTCACTATAGGG T7 promoter, forward primer T7 Terminal GCTAGTTATTGCTCAGCGG T7 terminator...GCAATTAACCCTCACTAAAGG T3 promoter, forward primer T7 TAATACGACTCACTATAGGG T7 promoter, forward primer Full Primer ...

  13. Promoters

    Type
    Guide
    ...Expression Description T7 Constitutive but requires T7 RNA polymerase Promoter from T7 bacteriophage Sp6 Constitutive...

  15. Modular Cloning Guide

    Type
    Guide
    ...Freemont 78 plasmids including constitutive promoters, T7 expression, RBS strength variants, synthetic terminators...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Grunwald pCS2TAL3-DD, pCS2TAL3-RR Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination...
Showing: 21 - 37 of 37 results