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We narrowed to 172 results for: TOX;

Showing: 21 - 40 of 172 results

  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...have the potential to form aggregates and may be toxic to the cell. Here comes the SunTag How does SunTag...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

  3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...important to note that the biotin-phenol reagent can be toxic to living cells thus APEX is not suitable for organoid...APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo were developed...rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent these issues, the...biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...live-cell imaging, the use of dyes that exhibit low toxicity and high photostability is crucial. These properties...dye's ability to enter cells without causing undue toxicity. A prominent example is DAPI, a dye that binds...in live cells can sometimes result in cellular toxicity due to the permeation process. Advanced technologies...of fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5)...

  5. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...recognized by effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells...programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of CTLs and NK cells. These cells would...immunotherapy. Using activated DCs ex vivo to generate cytotoxic effector T cells, and targeting specific DC subsets... targets like CD19 on tumor cells and exhibit cytotoxic activity. CAR-T cells have consistently shown ...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduction) targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate...cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations... by nature transient, which may lower nuclease toxicity or the potential for off-target effects. Third...

  7. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...replication in mammalian cells, for example, and a toxin that kills bacteria may not have any discernable... a cassette that will make cells resistant to a toxin), whereas negative selection would be the picking... a cassette that will make cells sensitive to a toxin). In the table below we focus on positive selection...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Long-term light exposure can also lead to cellular phototoxicity, and experimental success can be affected by...-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple subcellular...imaging, especially where photobleaching and phototoxicity are a concern, these constructs come in very...

  10. dTAG - You're it!

    Type
    Blog Post
    ...starting knock-in experiments. Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells...biological assay of choice to ensure that there are no toxicities at the doses used to evaluate degradation of ...have evaluated to date, we observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures...

  11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics listed ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy et al...

  14. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...genetic tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression...of FPs can trigger protein aggregation which is toxic for the cells and affects the labeling of small ...fluorescence. Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP...

  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...antigens found in the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs...tumor site. ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ...membrane, nucleus, etc.) are also available Non-toxic to living cells To learn more about this system,...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...

  20. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...fluorescence. Since emission of green light is more phototoxic to cells, most scientists working on in vivo ...fluorescence when exposed to blue light, which is less phototoxic to live tissue than the UV light used by other... a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their ...
Showing: 21 - 40 of 172 results