We narrowed to 43 results for: TYR
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TypeBlog Post...-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary inhibitory neurotransmitter...
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What's New in CRISPR - November 2021
TypeBlog Post...rearrangements as demonstrated in a mouse model of tyrosinemia, where a pathogenic insertion within the FAH ... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). The A260/230 ratio can be a good ... -
MXS Chaining
TypeBlog Post...Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...amino acids at the center of the protein (Ser65, Tyr66, and Gly67). When first discovered, one of the most... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...standby of CRE/flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating as they... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...editing event. This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage.” Molecular and Cellular Biology 5(... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...4060. https://doi.org/10.1038/sj.emboj.7600385 McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... reacts with electron-rich amino acids such as tyrosine on neighboring proteins resulting in their biotinylation... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar... Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina. Mol Ther... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...cell-type specific” promoter, such as the truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...medaka fish because its insertion into the fish’s tyrosinase gene caused albinism (Koga et al., 1996). Unlike... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M et al. 2018... -
27 Hot Plasmids from 2016
TypeBlog Post...164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All sequences and expression data were... -
AAV ddPCR Titration
TypeProtocol...1814040 Microseal adhesive seal, Bio-Rad, MSB1001 Polystyrene Reservoirs, VWR, 89094-662 Microcentrifuge tubes... the NTC. Pour the 1X dilution buffer into a polystyrene reagent reservoir. Using a 20–200 µL multichannel...cartridge. Add 800 µL of droplet generation oil to a polystyrene reagent reservoir. Using the 20–200 µL multichannel... -
Zhang Lab CRISPR Page
TypeCollection...cytomegalovirus (CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter... -
Lentivirus ddPCR Titration
TypeProtocol... Pierceable foil heat seal, Bio-Rad, 1814040 Polystyrene reservoirs, VWR, 89094-662 Microcentrifuge tubes...cartridge. Add 800 µL of droplet generation oil to a polystyrene reagent reservoir. Using the 20–200 µL multichannel... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4, VWR 45000... -
CRISPR Guide
TypeCollection...Rodríguez, T. C., Stan, T., Tysinger, E., Hong, L., Yudistyra, V., Ponnapati, M. R., Jacobson, J. M., Church...