Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 28 results for: TYR

Showing: 1 - 20 of 28 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...the eight mice born from Tyr Nanoblade treated zygotes, five had detectable Tyr editing at the phenotypic...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in albinism...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...have the option of including a previously-designed tyr gRNA, which causes hypopigmentation, thus marking...

  3. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...Some vendors take back their expanded polystyrene (a.k.a. Styrofoam or cooler boxes). We get these boxes...receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a landfill, but at least...with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in the U.S.). Their cooler boxes... 1976! Millipore Sigma takes back expanded polystyrene boxes for free (in the U.S.). After you receive...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...Instead they are detected with tyramide signal amplification. Tyramide signal amplification occurs when...when HRP converts a fluorescently labeled tyramide molecule into a highly reactive radical, which is then...Detection Method Uses Pros Cons Antigens HRP (P) Tyramide signal amplification of HRP Staining cultured...Enzymatic reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain with ...

  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ....013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs) are a major class of signaling ...the phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM...enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. bioRxiv 2022.08.13.503850...

  7. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...mRNA using 2a sites. These convert the amino acid tyrosine to betanin, a member of a class of pigments primarily... Fig. 2: (A) Betacyanin is produced from tyrosine by the activity of 3 enzymes and a spontaneous...

  8. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...glutamine insertion supF (tyrT)   Suppression of the amber (UAG) stop codon by tyrosine insertion λ-thi-1 ...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase (TH), can limit which cells express...mediated by three partial sequences of the human tyrosine hydroxylase promoter in vivo. Molecular Therapy...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... tools – light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large class ...

  11. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...to the office, however. We participate in many styrofoam and plastic recycling programs in the lab as well...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...covalent bond between the cleaved 3′ DNA strand and a tyrosyl residue of topoisomerase I (1). If a 5′ hydroxyl...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...rearrangements as demonstrated in a mouse model of tyrosinemia, where a pathogenic insertion within the FAH ...

  17. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm...
Showing: 1 - 20 of 28 results