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We narrowed to 48 results for: Tight TRE

Showing: 21 - 40 of 48 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... (TMP-tag) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that...cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility comes at ..., Article 4. https://doi.org/10.1038/nmeth749 Strebinger, D., Frangieh, C. J., Friedrich, M. J., Faure...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...its cap or aluminum foil (but do not make an air-tight seal!) and tape the bottle with autoclave tape. ...properly: Take out two plates. On the first plate, streak out a strain that you know to be resistant to the... the antibiotic. On the second plate, streak out a strain that’s not resistant to the antibiotic. Incubate...Strains Grow Assuming the appropriate strains were streaked on the appropriate plates, then if both strains...viable. You can check for this possibility by streaking out both strains on plates without any antibiotic...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yannick Doyon 214916 TRE-TDP-43ΔNLS-mClover3 TARDBP mClover3 TRE ALS Ophir Shalem 214917 TRE-mClover3-TDP-43ΔNLS...pEHA1628) SNCA Avi TRE Parkinson's Vikram Khurana 210360 TMEM106B-2Strep TMEM106B Strep CMV, T7 Frontotemporal...Flag CMV ALS Yossi Shiloh 34927 pTRE_tau-LacZ::tTA-H100Y MAPT LacZ TRE Parkinson's, FTD Mark Mayford 35000...telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart 44333 pMX_mTBP TBP LTR ...Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 ...Dent 188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off...Tet-off Full length Tau (0N4R, P301L/V337M) MAPT TRE Parkinson's, FTD Franz-Ulrich Hartl 188784 pBMN mEGFP-OPTN...

  4. Brain Initiative Collection

    Type
    Collection
    ...the hSyn1 promoter Viviana Gradinaru 117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper... through this effort will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...the contents. Tightly coupling barcodes and containers might not be such a bad idea, tightly coupling them...could be treating these pieces of data in ways that are inconsistent with how they are being treated in the...think carefully about how you want your software to treat those encoded numbers. Loosely couple containers...In the first example we’ve used inheritance to tightly couple the contents of a tube with the tube itself...

  6. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...drawbacks while considering this treatment as the duration of treatment can affect the cell growth and ... and antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods...media as much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid...exposure of the cell culture to air and make sure to tighten the flask cap before placing them in the incubators...agents can be used to wash the cell cultures. The treatment can take a few weeks to a couple of months depending...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...purple; sgRNA, dark blue.   The REC lobe binds tightly to the repeat:anti-repeat duplex of the guide RNA... It’s not actually clear! In vitro, Cas9 holds tightly to DNA even after cleaving it, so many researchers...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a specific ...

  10. Antibody Guide

    Type
    Collection
    ...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...creates variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to...Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope specificity...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ...isolate specific proteins or protein complexes for downstream analysis. They are powerful tools for understanding...Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations One type of ...

  11. Antibodies 101: ChIP

    Type
    Blog Post
    ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein... interest. Once the complex is captured, it is treated so that the protein and DNA are no longer bound... be isolated and studied through a variety of downstream methods, such as qPCR or next-gen sequencing.... the upsteam sample collection process or the downstream analysis process. And ChIP itself begins at.....between 200-1000 bp. This is to ensure that your downstream analysis focuses on the DNA your protein is actively...protein:DNA complexes. Your DNA is now ready for downstream analysis via any methods suitable for isolated...attach the proteins to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking...

  12. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent. This interplay...sides of the break, we determined that Cas9 held tightly to three of the four strands of the cleaved duplex...2014) have shown that this technique supports extremely high frequencies of genome cutting and, when donor...DNA is included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol...

  13. Antibodies 101: Conjugation

    Type
    Blog Post
    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical coupling...PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it...

  14. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression...eviction. Regulated Cre expression – placing Cre downstream of promoters that are active only in certain ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

  15. Working with Nuclear Receptors

    Type
    Blog Post
    ...transported from cells, traverse the body through the bloodstream, and eventually elicit their actions on other...drugs currently available or being developed to treat a multitude of therapeutic indices, including hypertension...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

  16. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving...expression can change as well. The more frequently and tightly bound DNA is to histones, the more compacted it...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...populations. Oftentimes, you’ll be presented with a trend of two populations with basically a smear in between..., it can depend on the type of stain whether a tighter or looser gate makes sense. Here, luckily, the ...

  18. To Each HIS Own

    Type
    Blog Post
    ... histidine residues that are present, the more tightly the protein will bind to the metal ion substrate...if you need a very pure sample. Because binding strength is proportional to the length of the tag, with...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    .... At this point, the plasmid DNA sample can be treated with RNase to remove RNA contamination and you ...nucleic acids. Containinants bind cellulose less tightly than nucleic acids, so placing the dipstick into...
Showing: 21 - 40 of 48 results