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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 25 results for: U6 gRNA

Showing: 21 - 25 of 25 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...for scientists to validate the efficacy of their gRNAs. The first step is to clone the target sequence ...ES) cells. These vectors, termed RUSH (For ROSA26 U6 short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30...of choice. Many of the available sgRNA-cargo constructs utilize a U6 promoter and contain the RNA cargo...targeted to their endogenous loci using validated gRNAs from Addgene and well designed homology directed...fused to an sgRNA used to direct Cas9 to a specific genomic locus. When fused to the sgRNA and co-expressed...dCas9 – the dCas9, sgRNA/RNA cargo complex localizes to the sequence targeted by the sgRNA. This allows scientists...machinery that brings the sgRNA/cargo RNA to the appropriate DNA locus. The sgRNA/RNA fusions retained their...construct. The sgRNA targeting Gaussia luciferase can be easily replaced with an sgRNA targeting your ...

  3. Promoters

    Type
    Guide
    ...Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...first position of the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced... for each sample and sequence each colony using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...primers flanking each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine...complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see step 1.4 ), 1.0 μl sgRNA 24- or 25-mer reverse...composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following...delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...
Showing: 21 - 25 of 25 results