..., it cannot be used for subcellular analysis such as morphology or subcellular localization studies. For...raised in the same host species, of the same Ig subclass, and conjugated to the same fluorochrome as your...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

... shown to be useful tools for light-mediated subcellular localization in mammalian cell culture and reversible...were used in a variety of experiments including subcellular visualization of 6 different organelles, a cell...dynamics. These fusion proteins have been used as subcellular markers to visualize the dynamics and interactions...proteins can act as markers for visualization of subcellular structure, for performing live-cell imaging experiments...

...transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3...bigger than ∼3 kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...

...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

... cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...

...lysosome-associated membrane protein, and following its subcellular localization overtime (Subach et al.) Monitor...particular biological event (trafficking to a subcellular location, start of gene expression, development...

... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

...eukaryotic) proteins localize to chemically distinct subcellular environments, including the organelles that compose...focused on optimizing fluorescent proteins for subcellular environment. Currently, she is a SPIRE postdoctoral...

...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments, like ...

...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...

... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...

...doing some research, notably determining the subcellular localization and the number of disulfide bonds...

...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...

...receptors are specific to isotype class or even subclass, and are part of the reason why different isotypes...