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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 770 results for: VARS

Showing: 21 - 40 of 770 results

  1. Fluorescent Proteins: FRET

    Type
    Collection
    ...donor and acceptor proteins fused with linkers of varying lengths. Description Article ECFP-EYFP FRET standards...standards, fusion proteins with linkers of varying length Evers et al. (2006) Quantitative understanding...and negative controls and cloning backbones for various FRET pairs with mTurquoise2 Mastop et al. (2017...brilliant and fast-maturing red fluorescent protein Variety of fusion proteins, positive controls for many ...

  2. Lentivirus Plasmids

    Type
    Collection
    ...miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion...fusion. See plasmids 36083 and 36086 for other FP variants. Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral...

  3. Cancer Research Plasmids and Resources

    Type
    Collection
    ...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling studies...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...

  5. TALEN Guide

    Type
    Collection
    ... a large number of zinc fingers that recognize various nucleotide triplets. With some trial and error,...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...31 repeats can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). ...

  6. Validated gRNA Sequences

    Type
    Collection
    ...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...gRNA activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with...elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...

  8. Rett Syndrome

    Type
    Collection
    ...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...enzyme responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic ...the protein is available, making it ideal for a variety of applications. To learn more and plan your experiment...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate...

  11. Bacterial Expression Systems

    Type
    Collection
    ...whose expression levels can be controlled by a variety of small molecules, light, and temperature, in ...#1000000037) contains specific bacterial promoters that vary in strength and that can be used to reliably drive...constructs. The Zinc Finger Consortium page contains a variety of reagents for engineering and expressing zinc...

  12. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Enhancers were evaluated in mouse brains, using various delivery methods including intracerebroventricular...contains best-in-class AAV plasmids to target a variety of cell populations in different brain regions...., G., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird,...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...expression, the system has been engineered with a variety of improvements. A synthetic Tet Response Element...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. To compare... General Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each ...

  15. Biosensor AAV Preps

    Type
    Collection
    ...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f...

  17. Brain Initiative Collection

    Type
    Collection
    ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...

  18. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

  19. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...
Showing: 21 - 40 of 770 results