... is: 5'-CACCGN...N-3' 3'-CN...NCAAA-5' You need to add the CACC and AAAC cloning overhang. When designing...

...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing...

...thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from various species PAM sequence...

...gene M13 Reverse CAGGAAACAGCTATGAC In lacZ gene M13/pUC Forward CCCAGTCACGACGTTGTAAAACG (Invitrogen) In...NOS-F GCGTTCAAAAGTCGCCTAAG Nopaline synthase promoter, forward primer Nmt1-F GCAATGTGCAGCGAAACTAA S. pombe...SV40-spliceR CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer T3 GCAATTAACCCTCACTAAAGG T3 promoter...to 3′. Commonly Used Primers CMV Forward CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter...of luciferase, reverse primer M13 Reverse CAGGAAACAGCTATGAC In lacZ gene MSCV CCCTTGAACCTCCTCGTTCGACC ...ATTTAGGTGACACTATAG SP6 promoter, forward primer T3 GCAATTAACCCTCACTAAAGG T3 promoter, forward primer T7 TAATACGACTCACTATAGGG... forward primer Full Primer List 3'AOX1 GCAAATGGCATTCTGACATCC (Invitrogen) For Pichia vectors with AOX1...

...thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from various species PAM sequence...

...can initiate. The TATA box is a DNA sequence (5'-TATAAA-3') within the core promoter region where general...